Psoriasis adalah penyakit kulit yang sering dijumpai, kronik, tidak menular, terjadi akibat kelainan kompleks pada pertumbuhan dan diferensiasi epidermal serta abnormalitas multipel dari biokimia, imunologi, dan vaskular. Salah satu faktor risiko psoriasis adalah fokal infeksi. Penelitian ini bertujuan mengetahui perbedaan tingkat keparahan psoriasis pada pasien psoriasis dengan dan tanpa disertai fokal infeksi di RSUD Prof. Dr. Margono Soekarjo, Purwokerto. Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross-sectional. Subjek penelitian adalah 40 pasien psoriasis yang berobat di Poli Kulit dan Kelamin RSUD Prof. Dr. Margono Soekarjo pada bulan November hingga Desember 2017 yang memenuhi kriteria inklusi dan eksklusi. Tingkat keparahan psoriasis diukur menggunakan Psoriasis Area Severity Index (PASI). Analisis data menggunakan independent t-test. Rata-rata tingkat keparahan psoriasis pada pasien dengan fokal infeksi 28,63 dan tanpa fokal infeksi 17,29, dengan jenis fokal infeksi didapatkan fokal infeksi intraoral (47,5%) dan genital (2,5%). Perbedaan tingkat keparahan psoriasis pada pasien dengan dan tanpa fokal infeksi didapatkan p = 0,000 (p < 0,05). Terdapat perbedaan signifikan tingkat keparahan psoriasis pada pasien dengan dan tanpa fokal infeksi.     Psoriasis is a common, chronic, noncontagious skin disease, caused by complex abnormal epidermal growth with multiple abnormality of biochemistry, immunology, and vascular. One of psoriasis risk factors is focal infection. This study was an analytical study with cross-sectional approach, to establish the difference of Psoriasis Area Severity Index (PASI) in patients with and without focal infections in Prof. Dr. Margono Soekarjo Public Hospital, Purwokerto. Subjects of this study were psoriasis outpatients in Dermatology and Venereology Clinic in November until December 2017. Forty subjects who fulfilled the inclusion and exclusion criteria were measured using PASI. This study showed the average of the PASI on patients with focal infections was 28.63 and without focal infections was 17.29, with types of the focal infection were intraoral focal infections (47,5%) and genital focal infections (2,5%). The difference of PASI between patients with and without focal infections was significant with p = 0,000 (p < 0,05). There is a significant difference on degree of psoriasis between patients with and without focal infections.

Background. Achatina fulica (A. fulica) snail slime was well known having many advantages especially as moisturizer and antimicrobial, but enzymatic antioxidant properties have not been studied yet. Objective. This study aimed to find the enzymatic antioxidant properties of snail slime extract and to find photoprotective potency in sunburn. Method. This research was an experimental test using randomized post-test only control group design using 75 male BALB/c mice were divided into 15 groups. There were an untreated group, 4 groups were given various doses of snail slime extract topically, 5 groups were irradiated with UVA 30J/cm2 and 5 groups were irradiated with UVB 200mJ/cm2. Skin biopsy were performed 24 hours after irradiated to be analyzed for enzymatic antioxidants properties of SOD, CAT and GPX, and also histologic changes. Data analysis were conducted with one-way ANOVA and followed by post hoc LSD with SPSS 26. Results. A. fulica snail slime contained enzymatic antioxidant properties of SOD, CAT and GPX respectively 40,59 + 5,79U/mL; 13,73 + 6,13 U/mL and 17,64 + 3,84 mmol/L. These antioxidant properties showed role of photoprotector compared to groups that had been radiated with UVA nor UVB. Topical use of snail slime extract significantly increased the level of SOD, CAT and GPX in sunburn model mice (p<0,05). This study also showed snail slime extract could prevent skin damage caused by UVA nor UVB histologically. Conclusion. Enzymatic antioxidants of A. fulica might have a photoprotector potency.

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BACKGROUND Psoriasis is an autoimmune disease with involvement of genetic and environmental factors. The HLA-Cw6 allele is the strongest genetic factor that correlates with earlier onset and more severe clinical manifestation of psoriasis. In Javanese ethnic, HLA-Cw6 allelic expression has not been identified, although increasing psoriasis patients were found in hospital dermatology departments. The study was aimed to determine the allelic expression of HLA-Cw6 based on the onset and severity of psoriasis in Javanese ethnic. METHODS A cross-sectional study was conducted from March to September 2018. Sixty psoriasis patients in the Margono Soekarjo Hospital and Banyumas Regional General Hospital were recruited consecutively. Psoriasis type, diagnosis, onset, and disease severity were assessed by dermatologists. HLA-Cw6 allelic expression was identified by DNA isolation, quantitative testing, and HLA-Cw6 genotyping. Fisher’s exact and chi-square tests were used to evaluate the association of HLA-Cw6 with age, clinical type, disease severity, onset, and gender. RESULTS The HLA-Cw6 allele positivity was identified at 297 base pairs (bp), while control showed at 618 bp bands. Among 60 subjects, 13 subjects (22%) showed HLACw6 positivity, and 47 subjects (78%) were HLA-Cw6 negative. HLA-Cw6 genotypes were similarly distributed concerning age, onset, gender, clinical type, and disease severity in psoriasis patients of Javanese ethnic (p > 0.05). CONCLUSIONS HLA-Cw6 expression type may not have influence to the characteristics of patients with psoriasis.

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