AIM OF STUDYTo study the clinical importance of reactive microenvironment in Hodgkin Lymphoma (HL) with special reference to macrophages and mast cells. MATERIALS AND METHODSThe present prospective and retrospective study was undertaken for a period ranging from January 2011 to June 2015 at the Department of Pathology, Kasturba Medical College, Mangalore. The Haematoxylin and Eosin (H and E) stained slides were reviewed and classified using WHO (2008) classification. Six immuno-histochemical markers were used in the study. CD 68 was for the macrophage count. Giemsa stain was done to highlight the mast cells. RESULTS AND ANALYSISThirty cases of HL were studied. Out of the 5 cases of Lymphocyte Depleted (LD) Classical Hodgkin Lymphoma (cHL), all cases showed high macrophage count. Out of 30 cases of HL, only 6 cases showed increased mast cell count. DISCUSSIONMast cells act actively in various types of cancers. They can either have a pro-tumorigenic function or an anti-tumorigenic function depending on the type of cancer. Four (80%) cases of LD-cHL showed macrophage count between 25-50% and 1 (20%) case showed macrophage count >50% correlating with the aggressive nature and advanced stage of the disease. CONCLUSIONIn this study of microenvironment of HL mast cells and macrophages were analysed in each subtype. Though the mast cells were seen in all cases, an increased count of >10/10 (High power field) HPF was observed only in 6 cases. The macrophage count was highest in LD-cHL and was statistically significant and thus correlated with this aggressive subtype of HL. The mast cell and macrophage count did not correlate with B-symptoms and stage of the disease a conclusion on survival versus the macrophage count and mast cell count was not possible in this study because of shorter follow up. A longer follow up and more number of cases are needed for a significant outcome.