Colicin E7 (ColE7) is a metallonuclease toxin of E. coli belonging to the HNH superfamily of nucleases. It contains highly conserved amino acids in its HHX14NX8HX3Hββα-type metal ion-binding C-terminal active centre. However, the proximity of the arginine at the N-terminus of the nuclease domain (NColE7,
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