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TX 75083-3836, U.S.A., fax 01-972-952-9435. AbstractCoiled tubing (CT) is widely used in well intervention as a practical and cost-effective means of servicing wells. Over the years, the actual flow through CT has been a point of discussion and theory. Testing has been conducted to promote a better understanding of what happens inside the CT. In recent years, the use of computational fluid dynamics (CFD) software has provided greater insight into actual CT flow patterns, including fluid-flow velocity profiles and secondary flow regimes. These flow patterns can be studied in a straight pipe or curved (CT) condition with variable flow rates and variable fluids. CFD could help us understand the phenomenon of erosion, including particle path and migration through the tubing. It might also lead to a greater understanding and efficient design of friction pressure gradients. After CFD is proven and established, an alternative to full-scale testing or other predictive methods may be possible.This paper discusses the flow phenomenon of CT using CFD, particularly over the tubing guide. This is an area of concern because the tubing configuration changes from straight to bent to straight again. Details of fluid flow for both the straight and curved sections have been examined to evaluate flow-velocity profiles and flow patterns. Fluids investigated have included water, gel, and slurry.

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This work provides information about bacterial community structure in natural wastewater treatment systems treating different types of wastewater. The diversity and composition of bacterial communities associated with the rhizosphere of Typha latifolia and Salix atrocinerea were studied and compared among two different natural wastewater treatment systems, using the direct sequencing of the 16S ribosomal RNA codifying genes. Phylogenetic affiliations of the bacteria detected allowed us to define the main groups present in these particular ecosystems.Moreover, bacterial community structure was studied through two diversity indices. Ten identified and five non-identified phyla were found in the samples; the phylum Proteobacteria was the predominant group in the four ecosystems. The results showed a bacterial community dominated by beta-proteobacteria and a lower diversity value in the swine wastewater treatment system. The municipal wastewater treatment system presented a high diverse community in both macrophytes (Typha latifolia and Salix atrocinerea), with gamma-proteobacteria and alpha-proteobacteria, respectively, as the most abundant groups.