The aim of the research was to study the correction of ossification processes and the mechanisms of humoral regulation of this process in the pathology of vitamin-mineral metabolism against the background of an immunosuppressive state in piglets. As a result of the experiment, it was found that proposed pharmacological correction scheme contributes to an increase in the level of total calcium (3.48 ± 0.29 mmol/l), ionized calcium (1.65 ± 0.07 mmol/l), ion-exchange calcium (2.52 ± 0.05 mmol/l), and protein-bound calcium (0.54 ± 0.06 mmol/l), 2, 3-diphosphoglycerate (1.43 ± 0.02 mmol/l), vitamin A (2.63 ± 0.09 μg/l), citric acid (2.90 ± 0.16 μg/l), a significant decrease in alkaline phosphatase activity (2.02 ± 0.04 mmol/l). Optimization of the structural and functional organization of the thyroid and parathyroid glands was noted, activation of hematopoiesis and bone tissue remodeling was observed in bone tissue, which suggests a pronounced reparative and osteoprotective effect of the proposed pharmacocorrection scheme.

The comorbidity of babesiosis and dirofilariasis in dogs is an important clinical problem, despite the significant achievements of recent years in understanding the pathogenesis of this mixed invasion. It has been established that the leading pathogenetic component in the development of the cytolytic syndrome with this comorbidity in dogs is oxidative stress resulting from the mismatch of the prooxidant and antioxidant resources of the cell under the influence of Babesia canis parasitism. On the basis of morphological, biochemical and ultrasonographic studies, a direct correlation was found between oxidative stress and hepatoprivial syndrome in dogs with comorbidity of babesiosis and dirofilariasis. Hepatoprivial syndrome was accompanied by the development of hypochromic anaemia, leukocytosis, hyperproteinemia, hypoglycemia, a disorder of pigment metabolism and an increase in the catalytic activity of serum enzymes, which indicated a violation of the metabolic activity of the liver and damage to its parenchyma. The activation of lipid peroxidation processes in the hepatocytes contributed to an increase in the catalytic activity of the blood serum enzymes in sick animals, and to a decrease in the antioxidant defence of sick dogs, due to a relative decrease in the level of vitamin A in the blood. Thus, the comorbidity of babesiosis and dirofilariasis in dogs enhances the oxidative syndrome that underlies the pathogenetic mechanisms of this mixed invasion, thereby increasing the degree of involvement in the pathological process of the liver, which is manifested by hepatoprivial syndrome. Keywords: oxidative stress, hepatoprivial syndrome, dirofilariasis, babesiosis, mixtinvasion, dogs

Activation of lipid peroxidation due to a disorder of redox homeostasis in dogs with comorbidity of babesiosis and dirofilariasis causes the involvement of not only the components of the hepatorenal system, but also the cardiopulmonary system in the pathological process against the background of the development of intoxication and hypoxic phenomena in hepatocytes and cardiomyocytes. As a result of the conducted biochemical studies of the blood of sick animals, it was found that the degree of redox homeostasis disorders directly correlates with the nature of morphofunctional disorders of the hepatorenal and cardiopulmonary systems, and the degree of these changes in the systems has an inverse correlation and is determined by the release of cytosolic enzymes into the blood. The involvement of the components of the hepatobiliary system in the pathological process is accompanied by a disorder of protein, carbohydrate, lipid and pigment metabolism.