Sexual selection may operate on pre-copulatory, copulatory, and post-copulatory traits. An example of a copulatory target of sexual selection is the genitalic movements a male performs during copulation. These movements may function either to prevent sperm competition or to influence a female's fertilization decision. Here we investigated how copulation duration, pedipalp movements, and abdominal movements that males of the pholcid spider Holocnemus pluchei produce during copulation influence sperm removal and/or patterns of successful sperm transfer. We compared mating events with virgin and mated females for differences in copulatory and post-copulatory behavior. We expected longer copulation duration, longer pedipalp movement duration, and more complex and frequent pedipalp and abdominal movements when males mated with mated females compared to virgin females. Except for abdominal movements, our results corroborated these predictions. Furthermore, when we investigated mating events with mated females, we observed sperm mass ejection from the female gonopore and physical removal of sperm by males' procursi. Females with interrupted second mating events showed a significant reduction of stored sperm masses compared to females with completed mating events. We suggest that males use alternating pedipalp movements to remove most of the rival sperm stored by mated females prior to sperm transfer. Copulation duration and pedipalp movements can be further used to transfer sperm and/or as a form of genitalic copulatory courtship.

BackgroundSexual conflict over mating rates may favour the origin and maintenance of phenotypes with contrasting reproductive strategies. The damselfly Ischnura elegans is characterised by a female colour polymorphism that consists of one androchrome and two gynochrome female morphs. Previous studies have shown that the polymorphism is genetic and to a high extent maintained by negative frequency-dependent mating success that varies temporally and spatially. However, the role of learning in male mating preferences has received little attention. We used molecular markers to investigate differences in polyandry between female morphs. In addition, we experimentally investigated innate male mating preferences and experience-dependent shifts in male mating preferences for female morphs.ResultsField and molecular data show that androchrome females were less polyandrous than gynochrome females. Interestingly, we found that naïve males showed significantly higher sexual preferences to androchrome than to gynochrome females in experimental trials. In contrast, experienced males showed no preference for androchrome females.ConclusionsThe ontogenetic change in male mate preferences occurs most likely because of learned mate recognition after experience with females, which in this case does not result in a preference for one of the morphs, but rather in the loss of an innate preference for androchrome females.

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The ‘functional allometry’ hypothesis proposes that the variation in allometric patterns of sexually selected traits is related to their function. We hypothesize that the allometric patterns for genitalia of aggressively territorial organisms are different from those in non-territorial organisms and predict that in aggressively territorial species, where body size is related directly to reproductive success, males must allocate more resources to body size than to genitalia. We studied 59 species of damselflies in 51 genera. Species were divided into three categories: highly territorial and aggressive; low aggressive; and not aggressive. We measured the length of the genital ligula, the width at the basis and its maximum width, and we used body length and wing length as descriptors of body size. The slope of allometric relationships was estimated using ordinary least squares and reduced major axis regressions. Our results indicated first, that territorial damselflies are larger and that body length and wing length are not equivalent as estimators of body size in odonates. Second, ordinary least squares and reduced major axis regressions provided different results in some of the analyses. Third, we found that aggressive species have less steep allometric slopes than non-aggressive species, both for the length of the ligula and for the width at its basis.

Artículo publicado en Open Access bajo los términos de Creative Commons attribution Non Comercial License 3.0. MONOGRÁFICO: Papel de los estudios comportamentales enEcología ecosistemas REVISTA CIENTÍFICA DE ECOLOGÍA Y MEDIO AMBIENTE