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Limonium algarvense Erben (sea lavender) is a halophyte species with potential to provide natural ingredients with in vitro antioxidant, anti-inflammatory, neuroprotective and antidiabetic properties. This study reports for the first time the 1) cultivation of sea lavender in greenhouse conditions under irrigation with freshwater (approx. 0 mM NaCl) and saline aquaculture wastewater (300 and 600 mM NaCl), and 2) the influence of the irrigation salinity on the plant performance (e.g. growth, number of produced leaves and flowers), in vitro antioxidant properties [radical scavenging activity (DPPH and ABTS), ferric reducing antioxidant power (FRAP), metal chelating properties on copper (CCA) and iron (ICA)], toxicity (in vitro on three mammalian cell lines) and chemical composition (determined by LC-ESI-HRMS/MS). The freshwater-irrigated plants had better growth performance than those irrigated with saltwater. Extracts from wild plants, had the highest antioxidant activity, but those from cultivated ones kept high in vitro antioxidant properties and interesting chemical profile. The flowers' extracts of plants irrigated with 300 mM NaCl had the highest antioxidant activities against DPPH, whereas those from freshwater-irrigated plants were more active on ABTS, CCA and FRAP. Most of the extracts showed nil toxicity. The flowers' extracts displayed the highest diversity of compounds, mainly quercetin, apigenin, luteolin, naringenin and their glycoside derivatives. Moreover, their abundance varied with the irrigation salinity. These data indicate that sea lavender plants can be successfully cultivated in greenhouse conditions under fresh-and saltwater irrigation, maintaining interesting biological and chemical properties. 1. Introduction Plants are used as a source of health improvement commodities since ancient times, generally as herbal infusions, juices, elixirs, and extracts (Miroddi et al., 2013). Nowadays, botanical nutraceuticals (e.g. raspberry ketones, green tea supplements, echinacea, Garcinia cambogia, Ginkgo biloba) are also used with the same purposes: to improve health, delay the aging process, prevent chronic diseases, increase life expectancy, and support the structure or function of the body (Nicoletti, 2012; Nasri et al., 2014). These products are sold in different forms, like fresh or dried products, liquid or solid extracts, tablets, capsules, powders, or tea bags (Grand View Research, 2017). Products containing natural ingredients, such as nutraceuticals, have generally easier access to consumers, lower prices and are more effective when compared to prescription drugs, which combined with the growing consumer awareness of the importance of a healthier lifestyle increased the popularity of these products and the demand for natural-based formulations (Nasri et al., 2014; Grand View Research, 2017). This boosted the need to identify and develop innovative and bioactive nutraceutical ingredients, sustaining the projections for the global nutraceuticals market of 578 billion dollars by 2025 (Grand View...

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The quality of the microalgae provided on Paracentrotus lividus larvae rearing is a primordial factor having a direct (nutritional properties) and indirect (water quality) impact on growth, competence and survival. Skeletonema costatum is a diatom commonly used in the bivalve cultivation. However, the use of this diatom in P. lividus larval cultivations is poorly known. The Rhodomonas spp. is a microalgae commonly used in sea urchin larvae culture. Three different diets were tested on P. lividus larvae and post-larvae cultivation (D1-Rhodomonas marina, D2-S. costatum, D3-mixture of both algae). Larvae fed with the D2 diet (55.8%) and D3 (39.9%) had a survival at 15 DAH higher than D1 (5.5%). The low survival in D1 could be due to the higher microbiological load on microalgae (Vibrio alginolyticus and V. pectenicide). Larvae fed with S. costatum (D2) showed a lower development than other diets. The competency index was lower for larvae fed with the D2.These results show that microalgae diversified diets contribute to a better development of P. lividus larvae. During the settlement and post-settlement phase, there was also a lower growth of the sea urchin fed with the D2 and a higher survival for D3.