The phytochemical  investigation of the stem bark extracts of Entada africana led to the isolation and characterization of Stigmasta-7,22-dien-3-ol from the dichloromethane soluble portion of acetone/methanol (1:1v/v) crude extract. The powdered stem bark sample of Entada africana was defatted with hexane and extracted with acetone/methanol (1:1v/v) mixture. The dichloromethane soluble fraction was purified on a low pressure column containing silica gel 60 (60-200 mesh). The purification afforded an isolate coded Enac3 (85 mg) with Rf value of 0.404 in hexane/ethyl acetate (4:1). The isolate was characterized using IR, NMR data and in comparison with literature. Analysis of spectroscopic data and literature comparison suggests Enac3 as stigmasta-7, 22-dien-3-ol. The isolation of stigmasta-7, 22-dien-3-ol from the stem bark of Entada africana suggests the presence of useful bioactive principles which could be exploited for medicinal purposes.

Investigation of the bioactive constituents of Entada africana crude extract afforded the isolation of alkylferulate. The hexane soluble portion of acetone/methanol (1:1v/v) crude stem bark extract of Entada africana was subjected to column chromatography on silica gel 60 (60-200) mesh size. Gradient column elution yielded an isolate coded AC4 with Rf value of 0.65 in hexane/diethyl ether (3:2). The isolate was characterized using IR, NMR and in comparison with literature data. The analysis of spectroscopic data and literature comparison strongly suggests that AC4 is an alkylferulate; a known hypolipidemic agent in addition to other biological uses. The isolation of alkylferulate partly lays credence to the use of Entada africana in traditional medicine practice.