A utilização de acaricidas sintéticos é a forma mais comum para o controle do carrapato-do-boi, Rhipicephalus (Boophilus) microplus. Porém, os problemas acarretados pelo o uso indiscriminado desses produtos têm impulsionado a busca por métodos alternativos como a utilização de compostos a base de plantas medicinais. Nesse contexto, este estudo objetivou avaliar a eficácia de diferentes métodos de extração do fumo de corda (Nicotiana tabacum) contra ovos de R. microplus. Para a obtenção dos extratos da planta, foram utilizadas as seguintes técnicas: maceração, destilação e extração de Soxhlet, todas utilizando a água como solvente. Os ovos foram organizados em alíquotas de 100 mg para serem tratados com os extratos e, posteriormente, observados diariamente para o registro dos parâmetros relativos à eclosão larval. Observou-se um aumento significativo no período de incubação quando foram utilizados os extratos obtidos por maceração ou Soxhlet. O período e o percentual de eclosão foram significativamente reduzidos, com a utilização de qualquer uma das técnicas de extração. Portanto, o extrato aquoso de N. tabacum, sem a necessidade de formulações, é eficaz para impedir a eclodibilidade larval sob as condições empregadas nesta pesquisa.

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Tick-borne pathogens belong to one of the two main groups of occupational biohazards, and occupational exposure to such agents puts soldiers at risk of zoonotic infections, such as those caused by rickettsiae. There are few studies on acarological fauna and occupational risk in military areas in Brazil. Thus, the present study aimed to analyze the diversity of ticks present in the military training areas of municipalities in the Southeast Region of Brazil. The ticks were collected from the selected areas using the dragging and flagging techniques as well as by visual detection on the operators’ clothing, and environmental information was also recorded. A total of ten species were collected from the 66 surveyed areas, belonging to five genera and nine species: Amblyomma sculptum, Amblyomma dubitatum, Amblyomma brasiliense, Amblyomma longirostre, Amblyomma aureolatum, Dermacentor nitens, Rhipicephalus spp., Ixodes spp. and Haemaphysalis spp. The frequent presence of tick species in military training areas along with traces and sightings of wild animals, most commonly capybaras (Hydrochoerus hydrochaeris), in most of the studied areas, indicates high levels of exposure of the military to tick vectors of spotted fever group rickettsiae and the possible occurrence of infections among the troops.

The present study aimed to use polymerase chain reaction (PCR) to detect species of the order Piroplasmida, such as Anaplasma spp., Borrelia spp., and Ehrlichia spp., circulating in the blood of Didelphis aurita in a peridomiciliary environment. Blood samples collected from big-eared opossum (Didelphis aurita) were screened for hemoparasites using PCR. The extracted DNA was tested for tick-borne hemoparasites. We were unable to detect hemoparasites, such as Ehrlichia spp., Babesia spp., Anaplasma spp., and Borrelia spp. Theileria DNA was detected in only one sample screened using PCR for an approximately 650-base pair fragment of the 18S rRNA gene. Sequencing and BLAST analysis of a subset of the PCR amplicons revealed 97% (535/553 bp) identity with Theileria bicornis. The detection of Theileria sp. in D. aurita challenges us to pursue more in-depth studies of marsupial piroplasmosids and to evaluate the morphological aspects of the findings and their possible involvement in zoonoses.