A method is described for the determination of dimethylnitrosamine in animal tissues. The tissue is homogenised with water and the homogenate is freed from protein by precipitation and filtration. A portion of the filtrate is made 3N with respect to sodium hydroxide and half is distilled. The dimethylnitrosamine in the distillate is determined polarographically, 0-01 M sulphosalicylic acid being used as the base solution. The minimum amount that can be determined is 1 pg, to an accuracy of kO.05 pg. Quantities smaller than 1 pg give ill-formed waves.The possibility of using the same technique for other nitrosamines is discussed briefly.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations鈥揷itations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.