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Lethality within food particles undergoing ohmic heating was investigated using microbiological and chemical marker measurements. Meatballs containing spores of B. stearothermophilus and precursors of chemical markers were thermally processed in a starch solution with 30–40% solids content using a 5 kilowatt (kW) ohmic system. Different temperatures, flow rates, holding tube lengths, and fluid electrical conductivities were used. Lethalities observed under various processing conditions were consistent with predictions. Higher lethality was observed, microbiologically and chemically, at the center of the meatballs rather than near the surface. A good correlation between the marker yield and the bacterial survivor population was obtained under various processing conditions. The results indicate that the chemical markers may be used to evaluate the distribution of lethality among different particles and the contribu‐

A method Is described for determining major constituents In the smoke of a cigarette that heats, but does not burn, tobacco. Dual, simultaneous separations are performed in a single gas chromatographic oven to determine water, glycerol, nicotine, and propylene glycol in a rapid and cost-effective manner. A materials balance of new cigarette smoke total particulate matter was attempted from both Cambridge filter and electrostatic precipitation smoke collection data. Serious deficiencies were found when Cambridge filter smoke collection was applied for this purpose. Electrostatic precipitation smoke collection eliminated these problems. The data obtained by electrostatic precipitation smoke collection Indicate that water, glycerol, nicotine, and propylene glycol make up about 94% of new cigarette smoke total particulate matter.