The electron paramagnetic resonance spectrum of Np~+ diluted in CszzrCi6 is analyzed in terms of the theory formulated by Berlorizky et a/. It is shown that if gj, the Lande g factor, is allowed to vary freely, a satisfactory fit may be obtained which is consistent with optical and EPR data obtained earlier on other tetrapositive actinide ions in the same host crystal. It is suggested that a better fit between the experimental data and the calculated spectrum may be obtained if the Zeeman mixing between the ground I 8 state and the excited I'6 state is considered.
REACTIONS depending on bacterial phosphatases have not hitherto been used for the identification of micro-organisms, principally because of difKculties in technique. Gordon and Cooper ( 1932) established the presence of glycerophosphatase in Bact. coli and staphylococci. Boivin and Mesrobeanu (1933) showed that Bact. wli and Staph. awe= have two phosphatase systems active a t pH 7 and 10. Heard and Wynne (1933) and Pett and Wynne (1933), using ground up organisms and glycerophosphate, hexosediphosphate and pyrophosphate as substrates, studied the phospbatase systems of CZ. acetobutyliczcm and Propioniboct, jensenii, and Pett and Wynne (1938) of Aerobacter aerogenes, Alkaligenes fmcalis and B. subtilis. Leahy, Sandholzer and Woodside (1939) demonstrated phosphatase in the " lyophilised " cells of various Cramnegative bacilli (Serratia, Pseudomonas, Escherichia, Aerobacter, Chromobacter, Proteus, Salmonella, Shigella, Eberthella and Bact. alkaligenes) using disodium phenyl phosphate as a substrate. Leahy, Stokinger and Carpenter (1940) showed that phosphatase was present in Neisseria. In all these investigations the cells employed were either non-viable or not multiplying.
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