Abstract-Measurement of the proliferative behaviors of in vitro cells is important to many biomedical applications ranging from basic biological research to advanced applications, such as drug synthesis, stem cell manufacturing, and tissue engineering. The detection of borders within an image constitutes a process of digitalization of the image. Once the digitized image is obtained, the next step is the application of a specific process consisting in applying algorithms that allow the obtaining of raw data of the image. In this case, the applied algorithm to the digitized images was the Canny algorithm. This work presents a system to compute a vector representation for a selected cell of an image. The representation is in bi-level raster image. Index Terms-Bi-level, cell tracking, raster image.