A universal primer (Sprimer: 5'-GGX AAY AAY AGY GGX CAZ CC-3', X = A, G, C or T; Y = T or C; Z = A or G), designed from the consensus sequences that code for the conserved sequence GNNSGQP in the NIb region of members of the family Potyviridae, was used to amplify by RT-PCR the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. Sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (ATTC PV-107) of Oat necrotic mottle virus is not a distinct species in the genus Rymovirus, but is synonymous with Brome streak mosaic virus (genus Tritimovirus) and that Celery mosaic virus is a distinct member of the genus Potyvirus not closely related to any other sequenced species. Potyviruses infecting crops in China were also investigated, showing that viruses on cowpea and maize in Hangzhou, Zhejiang province were respectively Bean common mosaic virus and Sugarcane mosaic virus and that one on garlic in Nanjing, Jiangsu province was Onion yellow dwarf virus. Fragments were also sequenced from Chinese isolates of Lettuce mosaic virus and Soybean mosaic virus (from Hangzhou), Turnip mosaic virus (2 different isolates from Zhejiang province) and RNA1 of Wheat yellow mosaic virus (from Rongcheng, Shandong province).

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To address the urgent need for high-capacity, scalable and energy-efficient data center solutions, we propose a novel data center network architecture realized by combining broadcast-and-select approach with elastic channel spacing technology.\ud We demonstrate that the proposed architecture is able to scale efficiently with the number of servers and offers lower energy consumption at a competitive cost compared to the existing solutions

Client-side optics are facing an ever-increasing upgrading pace, driven by upcoming 5G related services and datacenter applications. The demand for a single lane data rate is soon approaching 200 Gbps. To meet such high-speed requirement, all segments of traditional intensity modulation direct detection (IM/DD) technologies are being challenged. The characteristics of electrical and optoelectronic components, and the performance of modulation, coding and digital signal processing (DSP) techniques are being stretched to their limits. In this context, we witnessed technological breakthroughs in several aspects, including development of broadband devices, novel modulation formats and coding, and high-performance DSP algorithms for the past few years. A great momentum has been accumulated to overcome the aforementioned challenges. In this paper, we focus on IM/DD transmissions, and provide an overview of recent research and development efforts on key enabling technologies for 200 Gbps per lane and beyond. Our recent demonstrations of 200 Gbps short-reach transmissions with 4level pulse amplitude modulation (PAM) and discrete multitone signals are also presented as examples to show the system requirements in terms of device characteristics and DSP performance. Apart from digital coherent technologies and advanced direct detection systems, such as Stokes-vector and Kramers-Kronig schemes, we expect high-speed IM/DD systems will remain advantageous in terms of system cost, power consumption and footprint for short reach applications in the short-to mid-term perspective.

Degenerate primers were used to detect and amplify cDNA of viruses of the genera Carlavirus, Allexivirus and Potyvirus from garlic plants with mosaic symptoms growing in Zhejiang province, China. Plants contained a complex mixture of viruses and strains. Three distinct stains of Garlic latent virus were detected; the most frequent one was completely sequenced and partial sequences were obtained for the other two. The complete sequence (8363 nt) was 76.4% identical to a Korean isolate. Two allexiviruses were detected and completely sequenced. One (8319 nt) was identified as Garlic virus X and comparisons showed that a published Korean isolate (which had 90.2% identical nucleotides) had an N-terminal deletion in the serine-rich ORF4. The other isolate (8451 nt), tentatively named Garlic virus E, appeared to be a new member of the genus. Phylogenetic analyses of the different viral proteins and distinctive conserved sequence motifs within the genus are discussed. This is the first report of allexiviruses from China. Using potyvirus primers, three distinct isolates of Onion yellow dwarf virus and one of Leek yellow stripe virus were detected and the 3'-terminal sequences of their genomes were determined. In a coat protein phylogenetic analysis, the new isolates were most closely related to other published isolates from Japan and China.