No abstract

Hydrogenated diamond-like carbon (HDLC) has an atomically smooth surface that can be deposited on high-surface area substrata and functionalized with reactive chemical groups, providing an ideal substrate for protein immobilization. A synthetic sequence is described involving deposition and hydrogenation of DLC followed by chemical functionalization. These functional groups are reacted with amines on proteins causing covalent immobilization on contact. Raman measurements confirm the presence of these surface functional groups, and Fourier transform infrared spectroscopy (FTIR) confirms covalent protein immobilization. Atomic force microscopy (AFM) of immobilized proteins is reproducible because proteins do not move as a result of interactions with the AFM probe-tip, thus providing an advantage over mica substrata typically used in AFM studies of protein. HDLC offers many of the same technical advantages as oxidized graphene but also allows for coating large surface areas of biomaterials relevant to the fabrication of medical/biosensor devices.

In the present experimental work, we have described the signature of misoriented bilayer graphenelike and graphanelike structure in the hydrogenated diamond-like carbon film having interlayer disorder region and high specific surface area. Our new results have implications for bilayer graphene/graphane electronic devices.Index Terms-Graphane, graphite, high-field-effect mobility, hydrogenated diamond-like carbon (HDLC), interlayer disorder, misoriented bilayer graphene, Raman spectroscopy.