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IT has been reported (Laland, Dedichen, Thorsdalen, 1962: Dedichen, Laland, Laland andVoss, 1962) that materials prepared from ox liver and blood interfere with the growth of vaccinia virus in vivo and protect mice against Escherichia ccli infection. In the further study on the biological properties of such materials, the effect on the mitotic activity of HeLa cells and the Chang strain of normal liver cells in vitro has been examined. It has been found that these preparations inhibit mitotic activity. MATERIALS AND METHODSMaterials fromn liver. Twenty kg. of ox liver straight from a newly slaughtered animal was minced and mixed with 50 1. of water*, phenol added to a concentrationi of 0 5 per cent and the mixture heated under stirring for 30 minutes at 95'. The filtrate was concentrated in vacuum to 3 1. (containing 933 g. dry solid) and 5-3 1. of 96 per cent ethanol added. The mixture was filtered after 24 hours at room temperature and the filtrate concentrated in vacuum to 2-6 1. (8.5 ml.tri-cresol was added as a preservative).Two materials designated I and Vt have been prepared from this concentrate. 325 ml. of concentrate (equal to 2 5 kg. of liver) was extracted 8 times with 50 ml. of aqueous phenol (90 per cent) each time. The combined extracts were mixed with 6 volumes of ether and extracted 4 times with 25 ml. of water each time. The volume of the combined aqueous extracts was made up to 250 ml. with water, pH adjusted to 833 with 10 per cent KOH and 649 g. of Ba(CH3C00)2 added followed bv 1 1. of 96 per cent ethanol. The mixture was left at room temperature for 24 hours, the isolated precipitate was washed with ethanol and ether and dissolved in 150 ml. of water containing tri-cresol (0.3 per cent). The solution was passed through a columni of Amberlite IR 120 in the hydrogen form. The pH of the effluent was adjusted to 7 with aqueous ammonia (10 per cent), and the solution freeze-dried. 0 8 g. of a brownish yellow coloured material designated material I was obtained.

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