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Different methods have been devised to analyze vulnerability to cavitation of plants. Although a good agreement between them is usually found, some discrepancies have been reported when measuring samples from long-vesseled species. The aim of this study was to evaluate possible artifacts derived from different methods and sample sizes. Current-year shoot segments of mature olive trees (Olea europaea), a long-vesseled species, were used to generate vulnerability curves (VCs) by bench dehydration, pressure collar and both static- and flow-centrifuge methods. For the latter, two different rotors were used to test possible effects of the rotor design on the curves. Indeed, high-resolution computed tomography (HRCT) images were used to evaluate the functional status of xylem at different water potentials. Measurements of native embolism were used to validate the methods used. The pressure collar and the two centrifugal methods showed greater vulnerability to cavitation than the dehydration method. The shift in vulnerability thresholds in centrifuge methods was more pronounced in shorter samples, supporting the open-vessel artifact hypothesis as a higher proportion of vessels were open in short samples. The two different rotor designs used for the flow-centrifuge method revealed similar vulnerability to cavitation. Only the bench dehydration or HRCT methods produced VCs that agreed with native levels of embolism and water potential values measured in the field.

The compensation heat-pulse method for measuring sap flow is tested here in olive trees (Olea europaea L.). We describe a rigorous three-way examination of the robustness of the technique for this species, and examine the potential of the technique for an automatic control of the irrigation system. Two tests were carried out using heat-pulse gear inserted into the stem of 12-year-old 'Manzanilla' olive trees. One test used forced-flow through a stem section, and the other involved measured water uptake by an excised tree. The measured sap flow in these two tests was in agreement with calculations from heat-pulse velocities when using a standard 'wound correction' to account for the presence of the probes and the disruption to the sap flow. Thus, this technique for monitoring transpiration can, we feel, be used with confidence in olives. The third experiment was carried out in the field , where we analysed sap flow data from two 29year-old olive trees-one tree was under regular drip irrigation and the other was from dry-farming conditions. We use measurements of sap flow in the trunk to examine the hydraulic functioning of the tree, and to explore sorne diagnostics of water stress. Our heat-pulse measurements in the irrigated olive tree exhibited a profile of sap flow that was weighted towards the outer xylem of the tree trunk while the water-stressed trees in the field showed a profile of sap flow weighted towards the centre of the trunk. The loss of hydraulic functioning in the outermost section of the vascular system, as a result of water stress, we consider to be due both to stomatal control and to embolisms in the xylem vessels. The fourth experiment was also carried out in the field, in which sap flow measurements were made at three locations in the trunk as well as in two roots of another 29-year-old olive tree. The soil explored by each root, on opposite sides of the trunk, was differentially wetted by separate irrigation of each side. Our data showed that the surface roots were able to absorb water immediately after wetting, despite a reasonably prolonged period of moderate drought. Root activity quickly shifted to the regions where the soil had been wetted. A root in dry soil exhibited no flow at night, whereas sap flows of about 0.02 l h À1 were measured around midnight in the root drawing water from the wetter soil. Our observations suggest that the hydraulic behaviour of the trunk and surface roots might be used as a diagnostic of the onset, or severity, of water stress. Here there is not the imperative to replicate, for the prime goal is not transpiration estimation. Rather interpretation of the diurnal dynamics is used to infer the onset, or severity of water stress. The compensation heat-pulse seems a suitable technique for automatically controlling the irrigation system of olives, and probably other trees, based either on the estimation of the short-time dynamics of transpiration, or on changes in the hydraulic behaviour of the trees.

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SummaryIn plants with sclerophyll leaves, the response of stomatal and mesophyll conductance to CO2 to water stress and recovery is correlated with the expression of aquaporins and carbonic anhydrase.