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Cotton occupies sizeable area in India including Maharashtra which is progressively increasing with creation of irrigation amenities. Farmers adopting furrow irrigation causes percolation, nutrient leaching and volatilization at one end and unproductive loss of ground or surface water on the other end. Therefore, experiments were conducted during Kharif season of 2015-2016 to evaluate nutrient availability in Bt cotton by using drip fertigation under different phosphorous sources. The experiment consisted of eight treatments and replicated thrice. Application of 100% (24:24:00) fertigation through drip (T1)was significantly superior over soil application under surface irrigation and registered the maximum availability of N, P and K (241.11, 37.03 and 608.81 Kg ha-1) at 90DAS gradually it was decreasing at harvest stage and it consists 169.31, 19.97 and 459.81 Kg N, P and K ha-1. while comparing with surface irrigation treatment (T8) 5.4, 24.8 and 8.9% more availability of NPK in application of 100% fertilizer treatment (T1) at 90 DAS gradually it was decreasing because of fertigation was adopted up to 14 weekly splits.