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The influence of microorganisms from a salt marsh in the surface modification of 5083 aluminium alloy (Al-Mg) in seawater was evaluated. An immersion test performed for 50 days in biotic and abiotic conditions, with electrochemical monitoring and surface/cross-section characterization by SEM/EDX and TEM after exposure, showed that microorganisms induced the formation of a homogenous layer on the Al-Mg surface. This layer, which proved to be composed of a double-structure: a dense, amorphous inner layer and a more porous outer layer, was demonstrated to influence the corrosion resistance of the Al-Mg alloy in seawater.