Cortisol, which is produced in the adrenal glands, is an endogenous glucocorticoid hormone that delivers its hormonal message to cells by acting on glucocorticoid receptors. It is one of the main stress hormones responsible for stress responses in animals and humans, and its overproduction is characteristic of certain diseases. While acute stress disorder can be evaluated by means of measuring the cortisol concentration in blood and urine, chronic stress disorder can be detected by monitoring the cortisol concentration in fur or hair. Hair collection is simple, inexpensive and non-invasive, and can be performed easily and rapidly; thus, it appears to be a suitable method for determining the level of stress in dogs from shelters, abused dogs or dogs involved in different types of animal interactions. Since it is a relatively new method, monitoring cortisol in hair or fur requires further research in order to definitively prove its efficacy, and possibly to determine reference range values for different breeds of dogs.
Attention was devoted to the effects of risk element-containing industrial emissions on the biological material of cattle bred on farms in the vicinity of a metallurgical plant in East Slovakia. The observations lasted two years and included four agricultural farms in the polluted area and one control farm outside this area. In 1991, 20, 30, 30, 30, and 30 samples of milk, muscle, liver, heart, and kidney tissue were analyzed, whereas in 1992, 30, 48, 48, 48, and 48 respective samples were taken. For Cu, Zn, Mn, Cd, Pb, and Ni analyses, the AAS method was employed, using the UNICAM 939 device, while Hg residues were determined on the TMA 254 analyzer. In 1991, of a total of 120 samples of tissue and organs, 12.5%, 9.16% and 0.8% were found to contain above-limit levels of Cd, Ni, and Cu, respectively. The concentrations of the remaining elements, viz., Zn, Mn, Pb, and Hg, did not surpass the permitted maxima. In 1992, 1.03%, 2.60%, 0.51%, and 5.20% of 192 samples of tissue and organs contained above-limit Cd, Cu, Ni, and Zn levels.
Using a popular method of behaviour evaluation which rates the intensity of behaviour in different contexts, we demonstrate how pooling item scores relating to a given construct can reveal different potential risk factors for the dependent variable depending on how the total score is constructed. We highlight how similar simple total scores can be constructed through very different combinations of constituent items. We argue for the importance of examining individual item score distributions, and the results from different intensity thresholds before deciding on the preferred method for calculating a meaningful dependent variable. We consider simply pooling individual item scores which conflate context with intensity to calculate an average score and assuming this represents a biologically meaningful measure of trait intensity is a fallacy. Specifically using four items that describe intercat aggression and eleven that describe playfulness in cats in Fe-BARQ, we found sex and neuter status, social play and fearfulness were consistently significant predictors for intercat aggression scores; and age, age when obtained, social play and fearfulness were significant predictors of playfulness scores. However, the significance of other factors such as scratching varied with the threshold used to calculate to the total score. We argue that some of these inconsistent variables may be biologically and clinically important and should not be considered random error. Instead they need to be evaluated in the context of other available evidence.
Canine aggression is a widespread problem which receives a lot of attention from the media. It has important consequences in terms of public health and animal welfare. To address this issue it is necessary to first determine its epidemiological characteristics in the target population. This study was aimed at exploring the characteristics and determining the risk factors for dog aggression in the Slovak Republic and, in particular, for aggression directed at humans. For this purpose, we used a questionnaire directed to dog owners which included information about dog and owner characteristics, housing, training and fear behaviour. From the 177 analysed questionnaires, 67% portrayed dogs with some type of aggressive behaviour. Half of the animals showed some degree of owner-directed aggression and almost 40% were aggressive towards unfamiliar people. The approach of an unfamiliar male was the situation that stimulated most dogs to behave aggressively (33.3%) and a small percentage (2.3%) of the animals always showed aggression when approached by an unfamiliar child. Multivariate logistic regression models found that "owner's age" (OR, 0.96; 95% CI, 0.93 -0.99) and "neuter status" (OR, 0.36; 95% CI, 0.13-0.98) were significantly associated (P < 0.05) with the exhibiting of aggressive behaviour in different contexts. "Dog's sex" (OR, 2.16; 95% CI, 1.09-4.27) and "type of training" (OR, 2.32; 95% CI, 1.19-4.80) were significantly associated (P < 0.05) with the exhibiting of aggressive behaviour towards familiar people. Younger owners were more likely to have aggressive dogs and neutered dogs were less likely to be aggressive in different contexts. Male dogs and dogs with informal training were more likely to be aggressive towards familiar people. Manifestations of fear were significantly associated with the expression of aggressive behaviour in all models. This study provides the first preliminary results on the characteristics and potential risk factors for human-directed aggression in Slovakia. The analysis of these data may help in determining which preventive measures should be given priority in this country.
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