The viability of the microbes Saccharomyces cerevisiae, Bacillus circulans, Escherichia coli, Micrococcus luteus, Pseudomonas fluorescens, Salmonella enteritidis, Serratia marcescens, and Staphylococcus aureus was tested under static magnetic field exposure up to 24 h in either a homogeneous (159.2 +/- 13.4 mT) or three types of inhomogeneous static magnetic fields: (i) peak-to-peak magnetic flux density 476.7 +/- 0.1 mT with a lateral magnetic flux density gradient of 47.7 T/m, (ii) 12.0 +/- 0.1 mT with 1.2 T/m, or (iii) 2.8 +/- 0.1 mT with 0.3 T/m. Even the longest period of exposure failed to produce any effect in the growth of bacteriae that could be correlated with static magnetic field exposure.

A Gram-reaction-negative halotolerant bacterial strain, designated Ka21T, was isolated from agricultural soil and characterised using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, highest similarity was found with Sphingobacterium alkalisoli Y3L14T (96.72%). Cells were observed to be aerobic, non-motile rods. The isolate was found to be able to grow between 0 and 10% of NaCl concentration. The assembled genome of strain Ka21T has a total length of 5.2 Mb with a G + C content of 41.0 mol%. According to the genome analysis, Ka21T encodes several glycoside hydrolases that may play a role in the degradation of accumulated plant biomass in the soil. Based on phenotypic characteristics and phylogenetic analysis, it is concluded that strain Ka21T represents a novel species in the Sphingobacterium genus for which the name Sphingobacterium pedocola sp. nov. is proposed. The type strain of the species is strain Ka21T (= LMG 31575T = NCAIM B.02636T).

No abstract

No abstract

The oxygen utilization properties of different commercially available yeast (Saccharomyces cerevisiae) products were tested in vitro in artifi cially created ruminal environment. It has been established that different yeast strains have different oxygen consuming capacity and this characteristic changes in case of prior anaerobic incubation. It was shown that Live-Sacc ® (NCAIM deposited strain) is a powerful fermentative, it quickly extracts O 2 from the rumen fl uid and the anaerobic conditions have no effect on its oxygen consuming capacity.