By means of studying project management body of knowledge and analyzing characteristics of R&D enterprise project management, the differences between R&D enterprise project management and the current construction project management in management content, process and methods are explored, the view that R&D project management process consists of knowledge innovation and knowledge management is emphasized, and then knowledge management in R&D project management is discussed, knowledge management methods in R&D enterprise project are studied, and the view that creating standardized processes and technical documentation templates, establishing knowledge management information system and constructing a knowledge sharing culture are efficient ways to solve knowledge management is put forwarded. Furthermore, in conjunction with the practice of R&D enterprise information system, the view that knowledge management-based project management system is an effective approach is confirmed.