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Salmonella enterica serotype Enteritidis is an important alimentary pathogen that recently gained special attention due to the ability of a large number of strains to form biofi lms. Qualitative testing of biofi lm forming ability was performed by observing the morphotype of the colonies on Congo Red agar and by conducting the pellicle test, while quantitative testing was carried out by Cristal violet assay on microtiter plates. A total of 14 isolates of S.Enteritidis were tested for biofi lm forming ability, while Salmonella Enteritidis ATTC 13076 was used as the reference strain. Based on the morphotype of colonies cultivated on Congo Red agar at 25°C incubation temperature, among tested isolates three morphotypes were detected -red, dry and rough (rdar), brown, dry and rough (bdar) and smooth and white (saw). Half of the tested isolates demonstrated rdar morphotype. All isolates that showed a specifi c morphotype at this incubation temperature also formed the corresponding type of pellicle at the air-liquid interface. Additionally, comparing OD (optical density) values obtained by crystal violet test between groups of isolates that represent one of the three detected morphotypes (rdar, bdar and saw), statistically signifi cant differences were detected. Based on OD values obtained by crystal violet test at both applied incubation temperatures, isolates were classifi ed into three categories, regarding their ability to form biofi lms: strong, moderate and weak biofi lm producers. By comparing the amounts of the biofi lms formed after 48h at 25°C and 37°C, statistically signifi cant differences were noted (P<0.05). In this research we presented micrographs and a reconstruction of threedimensional projections of biofi lm developing phases of rdar morphotype isolates, which were obtained using confocal laser scanning microscopy.

This paper investigates the effect of extruded rapeseed grain on production parameters, carcass and breast meat quality of broiler chicken. There were three levels mixture of extruded rapeseed grain and corn meal used included with 10, 15 and 20%. Rapeseed grain is extruded with corn meal in ratio of 50:50%. At the beginning of the fattening period, four groups with 75 day-old chicks of hybrid Ross 308 were formed in four replications. The experiment lasted for 42 days. At the end of the experimental period, eight chickens from each group were sacrificed for the purpose of testing the carcass quality and breast meat of broilers. Inclusion of various amounts of this mixture in the diet for broilers resulted in a statistically significant difference in body weight of chickens. At the end of the experiment, control group achieved body weight of 2115.80 g and the experimental groups in a row, 2018.17, 2036.49 and 2047.32 g respectively. Feed conversion was increased from 2.5 to 3.5% in groups with extruded rapeseed grain. Partial substitution of soybean meal with extruded rapeseed grains did not significantly affect the carcass quality of chickens, or the nutritional quality of breast meat.

No abstract

The possibilities to use microbial phytase in maize/soybean meal diets for broilers with different dietary total and available phosphorus (TP and AP) levels were investigated. A 21-day experiment was carried out on 300 Arbor Acres broilers of both sexes divided into six groups. The broilers were fed diets with 0.46%, 0.25% or 0.10% AP, with or without phytase supplementation. During the experiment, health status and mortality, performance and physical, chemical and morphological bone tissue characteristics were investigated. Total exclusion of a mineral source of P from the diet increased mortality and health problems. Addition of phytase decreased mortality and alleviated negative effects of dicalcium phosphate exclusion. Reduction of dietary TP and AP level induced lower body mass and daily gain and feed consumption, as well as higher feed conversion. However, by introducing phytase into diets negative effects of P reduction were, to some extent, alleviated. Physical, chemical and histological analysis of broiler tibia indicates that the extent and significance of changes depend on P deficiency and phytase addition. Phytase efficacy was greater in diets with a reduced level of dicalcium phosphate