The γ-ray sky can be decomposed into individually detected sources, diffuse emission attributed to the interactions of Galactic cosmic rays with gas and radiation fields, and a residual all-sky emission component commonly called the isotropic diffuse γ-ray background (IGRB). The IGRB comprises all extragalactic emissions too faint or too diffuse to be resolved in a given survey, as well as any residual Galactic foregrounds that are approximately isotropic. The first IGRB measurement with the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope (Fermi) used 10 months of sky-survey data and considered an energy range between 200 MeV and 100 GeV. Improvements in event selection and characterization of cosmic-ray backgrounds, better understanding of the diffuse Galactic emission (DGE), and a longer data accumulation of 50 months allow for a refinement and extension of the IGRB measurement with the LAT, now covering the energy range from 100 MeV to 820 GeV. The IGRB spectrum shows a significant high-energy cutoff feature and can be well described over nearly four decades in energy by a power law with exponential cutoff having a spectral index of 2.32 ± 0.02 and a break energy of (279 ± 52) GeV using our baseline DGE model. The total intensity attributed to the IGRB is (7.2 ± 0.6) × 10 −6 cm −2 s −1 sr −1 above 100 MeV, with an additional +15%/−30% systematic uncertainty due to the Galactic diffuse foregrounds.

Ca decorated carbon allotropes have a potential for high density hydrogen storage, except that the CaÀgraphene and CaÀfullerenes binding is not strong enough to prevent the formation of a Ca cluster. Using first-principles calculations, we show that Ca can bind strongly to sÀp and sÀp 2 bonded graphyne without the formation of a Ca cluster. This enhanced binding energy is due to the additional in-plane π states which do not exist in the sÀp 2 bonded graphene and fullerenes. The H 2 binding to the CaÀgraphyne system is similar to the CaÀfullerenes system with a maximum of six H 2 molecules per Ca atom and a 0.2 eV per H 2 binding energy which is optimal as hydrogen storage materials. With two Ca atoms per unit cell, this leads to 9.6 wt % hydrogen storage capacity in theory.

Substrate inhibition of enzymes can be a major obstacle to the production of valuable chemicals in engineered microorganisms. Here, we show substrate inhibition of lycopene cyclase as the main limitation in carotenoid biosynthesis in Yarrowia lipolytica. To overcome this bottleneck, we exploit two independent approaches. Structure-guided protein engineering yields a variant, Y27R, characterized by complete loss of substrate inhibition without reduction of enzymatic activity. Alternatively, establishing a geranylgeranyl pyrophosphate synthase-mediated flux flow restrictor also prevents the onset of substrate inhibition by diverting metabolic flux away from the inhibitory metabolite while maintaining sufficient flux towards product formation. Both approaches result in high levels of near-exclusive β-carotene production. Ultimately, we construct strains capable of producing 39.5 g/L β-carotene at a productivity of 0.165 g/L/h in bioreactor fermentations (a 1441-fold improvement over the initial strain). Our findings provide effective approaches for removing substrate inhibition in engineering pathways for efficient synthesis of natural products.