The present investigation entitled "Effect of Plant Growth Regulators on Rooting and Growth of Grape Rootstock" was undertaken at the Instructional-cum-Research Farm, College of Agriculture, Osmanabad, Dist. Osmanabad. The experiment was laid out in Factorial Randomized Block Design (FRBD) with two factors first factor was Rootstock Variety with five level viz. Dogrigde, Salt Creek, 1613 C, 110 R and 99 R and second factor was plant growth regulators level (IAA-100ppm, IAA-200 ppm, IBA-100 ppm and IBA-200 ppm). Total 20 treatment combinations tried were replicated thrice. The cuttings of rootstocks were treated with plant growth regulators as per treatments and it was conducted in first week of November 2019. The observations on root character of grape rootstock were recorded. The root characters of grape rootstock were significantly influenced by different level of rootstock variety and plant growth regulators. The results revealed that all the root character of grape rootstock cutting i.e. Maximum callus diameter (32.99 mm), Rooting percentage (74.03 %), length of longest root (20.11), number of primary roots (19.00) and root diameter (1.11 mm) were noted under the treatment T 9 . The significantly maximum fresh weight of root (1.66 g) and dry weight of root (0.89 g) were noted under the treatment T 1 . So, it can be concluded from above study that, for better rooting performance the plant growth regulator (IAA) with concentration (100 ppm ) is suitable.

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