Anti-cancer activities of chloroform and hydro-alcohol leaf extracts of Bambusa bambos was evaluated in vitro using Dalton’s Lymphoma Ascites (DLA) and Ehrlich’s Ascites Carcinoma (EAC) cell lines by Trypan blue dye exclusion method. The chloroform extract exhibited better activity compared to hydro-alcohol extract. Further hemolytic activities of both the extracts were carried out to measure the extent of damage to normal red blood cell membranes. The findings suggested that both the extracts produced no signs of hemolysis indicating that the extracts are not toxic to normal erythrocytes