1Drought is an important factor reducing yield and quality of sugarcane. Root growth and 1 2 physiological traits are important for maximizing water uptake to improve drought resistance. 3This study compared the root, shoot and physiological traits under drought stress (DS) and 1 4 well-watered (WW) conditions of various sugarcane varieties grown in rhizoboxes in a 1 5 greenhouse at the Field Crops Research Station of Khon Kaen University, Khon Kaen, 1 6Thailand. Data were recorded for the root, shoot and physiological traits (relative water 1 7 content, stomatal conductance, SPAD chlorophyll meter reading and chlorophyll 1 8 fluorescence) at 90 days after transplanting. Root samples were recovered from 11 soil layers 1 9 3 0 3 1

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