Nitrogenase activity (by acetylene reduction assay) has been found on the roots and in the rhizosphere‐soil of various plant species collected from dune soils (Table 1.) located on the west coast of Denmark. Out of twelve plant species, five plant species (Deschampsia flexuosa, Ammophila arenaria, Calluna vulgaris, Empetrum nigrum and Corynephorus canescens) revealed a positive nitrogenase activity. Among these five, three were temperate grasses. Except Ammophila arenaria, all four plant species were collected from old sand dune and showed nitrogenase activity on roots as well as in their rhizosphere soil, Ammophila arenaria was collected from fresh sand dune and exhibited nitrogenase activity only on roots (Table 2).