Whole kernel lysine content was determined on inbred lines and Plant Introduction strains of corn (Zea mays L.). The lysine values ranged from 0.16 to 0.45%. The largest values are comparable to whole kernel lysine contents of opaque‐2 mutants. The distribution was normal with a mean of 0.30% and a standard deviation of 0.04%. A significant correlation (r=0.48) was obtained between whole kernel lysine and protein for a selected group of high‐lysine lines. Little variability in lysine content was noted for six inbred lines grown at different stations in different years.