Single-molecule force spectroscopy using an atomic force microscope (AFM) can be used to measure the average unfolding force of proteins in a constant velocity experiment. In combination with Monte Carlo simulations and through the application of the Zhurkov-Bell model, information about the parameters describing the underlying unfolding energy landscape of the protein can be obtained. Using this approach, we have completed protein unfolding experiments on the polyprotein (I 27) 5 over a range of pulling velocities. In agreement with previous work, we find that the observed number of protein unfolding events observed in each approach-retract cycle varies between one and five, due to the nature of the interactions between the polyprotein, the AFM tip, and the substrate, and there is an unequal unfolding probability distribution. We have developed a Monte Carlo simulation that incorporates the impact of this unequal unfolding probability distribution on the median unfolding force and the calculation of the protein unfolding energy landscape parameters. These results show that while there is a significant, unequal unfolding probability distribution, the unfolding energy landscape parameters obtained from use of the Zhurkov-Bell model are not greatly affected. This result is important because it demonstrates that the minimum acceptance criteria typically used in force extension experiments are justified and do not skew the calculation of the unfolding energy landscape parameters. We further validate this approach by determining the error in the energy landscape parameters for two extreme cases, and we provide suggestions for methods that can be employed to increase the level of accuracy in single-molecule experiments using polyproteins.

An apparent gap in the Andean volcanic arc in the Pampean section of the subduction zone in Chile (~28°-33°S) marks a section of flat slab subduction. In this tectonic environment, the fate of fluids released from the subducting Nazca slab remains uncertain and the degree of their interaction with the basal layer of the continental lithosphere is poorly understood. Results of a RF investigation and forward modeling effort at three long-running stations of the Chilean National Seismic Network allow us to constrain the position of the subducting Nazca slab and to address the physical properties of the interplate contact zone in Central Chile. Our observations suggest a transition in seismic character, from a weakly anisotropic contact in the normally subducting section north of the flat slab region to a strongly anisotropic plate contact within the flat slab region. We attribute this change to a transition from sheared olivine to serpentinized peridotite generated as a result of fluid release across the flat slab. This interpretation is supported by forward modeling synthetic RFs at each of the stations. We propose that the identified layer extends across the flat slab region, acting as a mineral reservoir that captures and, possibly, transports fluids from the dehydrating Nazca Plate as it subducts below South America. We note that the Ps converted phase at the slab interface at southernmost station GO04 suffers a 2-s discontinuity at 180°back azimuth, consistent with a 15-20-km scarp or kink in the Nazca slab to the south of the station.

The recycling of Earth materials through subduction is the driving force of plate tectonics. The downgoing oceanic lithosphere can be of diverse nature with regimes of over-thickened crust along ridges, seamounts, and transform boundaries that alter composition, thickness, and relief of the plate (Stern, 2002). These features impact the dynamics of the subduction process and have significant influences on the Earth's surface expression (e.g., Morell et al., 2016). A particularly complex tectonic environment can be observed in southern Costa Rica where the nature of subducted materials dramatically changes over a distance of ∼500 km from the typical oceanic crust produced at the East Pacific Rise to seamounts and the ∼20-km-thick Cocos Ridge derived from the Galapagos Hot Spot track, and the Panama Fracture Zone (PFZ) transform boundary (Figure 1). The surficial expression of changes in the nature of the downgoing slab can be noticed from northern to southern Costa Rica. Near-orthogonal subduction of typical oceanic lithosphere from the East Pacific Rise

Surrounded by subducting slabs and continental keels, the upper mantle of the Pacific is largely prevented from mixing with surrounding areas. One possible outlet is beneath the southern part of the Central American isthmus, where regional observations of seismic anisotropy, temporal changes in isotopic composition of volcanic eruptions, and considerations of dynamic topography all suggest upper mantle flow from the Pacific to the Caribbean. We derive new constraints on the nature of seismic anisotropy in the upper mantle of southern Costa Rica from observations of birefringence in teleseismic shear waves. Fast and slow components separate by ~1 s, with faster waves polarized along the 40°–50° (northeast) direction, near-orthogonally to the Central American convergent margin. Our results are consistent with upper mantle flow from the Pacific to the Caribbean and require an opening in the lithosphere subducting under the region.

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