Recent studies have suggested that alveolar nitric oxide (NO) concentration is a noninvasive test of distal lung inflammation.The current study determined whether alveolar NO concentration can be measured in patients with asthma of varying severity, tested the hypothesis that there is an association between alveolar NO and bronchoalveolar lavage (BAL) eosinophil count and determined whether refractory asthma is characterised by a raised alveolar NO concentration. Finally, the present authors assessed the effect of 2 weeks of prednisolone (30 mg q.d.) on alveolar NO concentration.Alveolar NO concentration was both measurable and repeatable in patients with refractory asthma. A positive correlation was found between alveolar NO concentration and BAL eosinophil count but not with bronchial wash or sputum eosinophil count. Alveolar NO concentration was increased in patients with refractory asthma (7.1 ppb) compared with mildto-moderate asthma (3.4 ppb) and normal controls (3.4 ppb) and reduced by treatment with prednisolone.In conclusion, these findings support the hypothesis that alveolar nitric oxide is a measure of distal airway inflammation and suggest that distal lung inflammation is present in refractory asthma.

During clathrin-mediated endocytosis, dozens of proteins assemble into an interconnected network at the plasma membrane. As initiators of endocytosis, Eps15 and Fcho1/2 concentrate downstream components, while permitting dynamic rearrangement within the budding vesicle. How do initiator proteins meet these competing demands? Here we show that Eps15 and Fcho1/2 rely on weak, liquid-like interactions to catalyze endocytosis. In vitro, these weak interactions promote the assembly of protein droplets with liquid-like properties. To probe the physiological role of these liquid-like networks, we tuned the strength of initiator protein assembly in real time using light-inducible oligomerization of Eps15. Low light levels drove liquid-like assemblies, restoring normal rates of endocytosis in mammalian Eps15 knockout cells. In contrast, initiator proteins formed solid-like assemblies upon exposure to higher light levels, which stalled vesicle budding, likely owing to insufficient molecular rearrangement. These findings suggest that liquid-like assembly of initiator proteins provides an optimal catalytic platform for endocytosis.

To some extent the theoretically proposed five-factor structure of the Norwegian version of the SDQ self-report was supported in 15- to 16-year-old adolescents. However, the results of more detailed analyses raise questions about the interpretation of some subscales. When applying this screening method to Norwegian adolescents, our results suggest that the use of the total difficulty score of the SDQ in screening youth should be preferred over the subscale scores.

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A solid oxide fuel cell (SOFC) is a solid-state energy conversion system that converts chemical energy into electrical energy and heat at elevated temperatures. Its bipolar cells are electrochemical devices with an anode, electrolyte, and cathode that can be arranged in a planar or tubular design with separated gas chambers for fuel and oxidant. Single chamber setups have bipolar cells with reaction selective electrodes and no separation between anode and cathode compartments. A nickel/yttria-stabilized-zirconia (YSZ) cermet is the most investigated and currently most widespread anode material for the use with hydrogen as fuel. In recent years, however, doped ceria cermet anodes with nickel or copper and ceria as the ceramic phase have been introduced together with ceria as electrolyte material for the use with hydrocarbon fuels. The state-of-the-art electrolyte material is YSZ of high ionic and nearly no electronic conductivity at temperatures between 800-1000 °C. In order to reduce SOFC system costs, a reduction of operation temperatures to 600-800 °C is desirable and electrolytes with higher ionic conductivities than YSZ are aimed for such as bismuth oxide, lanthanum gallate or mixed conducting ceria and the use of thin electrolytes. Proton conducting perovskites are researched as alternatives to conventional oxygen conducting electrolyte materials. At the cathode, the reduction of molecular oxygen takes place predominantly on the surface. Today's state-of-the-art cathodes are La x Sr 1-x MnO 3-d for SOFC operating at high temperature i.e. 800-1000 °C, or mixed conducting La x Sr 1-x Co y Fe 1-y O 3-d for intermediate temperature operation, i.e. 600-800 °C. Among the variety of alternative materials, Sm x Sr 1-x CoO 3-d and Ba x Sr 1-x Co x Fe 1-x O 3-d are perovskites that show very good oxygen reduction properties. This paper reviews the materials that are used in solid oxide fuel cells and their properties as well as novel materials that are potentially applied in the near future. The possible designs of single bipolar cells are also reviewed.