In a previous paper (1) it was shown that triton WR-1339, I a non-ionic surface-active agent, produces, in vitro, notable physical and chemical changes of serum lipoproteins and chylomicrons. I t was assumed that if similar alterations occurred in vivo, they might be partially responsible for the hyperlipemia that had been described in rabbits (2), guinea pigs (2, 3), mice (4), and rats (5) following administration of the detergent. To test this assumption, 14 dogs were given prolonged intravenous treatment with triton. Overt hyperlipemia developed in all animals, and they died within 4 months after receiving the first injection of the detergent. The changes in plasma lipids and lipoproteins and the clinicopathologic findings will be described. Methods and ProceduresAnimals and D/et.--The animals used were male mongrel dogs, from 1 to 2 years of age, which weighed between 9 and 14 kg. They were kept in single cages and were consistently fed a diet that was 61 per cent carbohydrate, 32 per cent protein, and 7 per cent fat. The amount of food offered daily furnished about 1300 calories, 2 195 calories or 15 per cent of which was due to fat. Blood samples, for chemical analyses, were drawn from the femoral vein weekly, after a fasting period of about 12 hours.

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Dogs fed a regular diet were given intravenously 250 mg of Triton WR-1339 (a nonionic surface active agent) every 4th day. When sustained lipemia developed, their plasma free fatty acid (FFA) response to epinephrine and glusose was studied and compared with that of normal animals. The time course of the rise (epinephrine) or fall (glucose) of the plasma FFA was similar in the two groups of animals. The Triton-treated dogs had, however, plasma FFA levels twice as high as normals. Plasma FFA, which in control dogs were bound principally to albumin, were mostly associated in the Triton-treated animals with the low-density ß-lipoproteins. A partial shift of FFA from albumin to ß-lipoproteins could be produced in vitro by addition of Triton to normal canine serum.

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