No abstract

Infrared spectroscopy is well established as an analytical technique in various applications. We have undertaken a series of studies to establish the suitability of mid-infrared spectroscopy in various clinical analytical applications, focusing on various urine, serum and whole blood assays. The initial work demonstrated that six common serum analyses are possible, namely glucose, urea, total cholesterol, triglycerides, total protein, and albumin, with accuracy comparable to standard clinical methods (Hitachi 717), and more recently HDL and LDL cholesterol have been quantified separately. Herein, we summarize our progress in transferring this technology to the clinical laboratory, focusing on the new methods and hardware that have enabled this transition, assessing the accuracy of the mid-IR-based analytical methods using these innovations, and reporting an exploratory study assessing the transferability of methods between spectrometers.