With each group was placed one of the nonvaccinated control heifers, each group of three occupying an isolated stall. To test the resistance conferred by the vaccine, virulent bovine tubercle bacilli from cultures were weighed in a fresh state and inoculated into 19 5 ' of the heifers, intravenously, in 5-mg doses with the following results: Group I. Vaccinated heifers 1 and 2 and control No. 13 were inoculated 1 month after vaccination. Control No. 13, killed 60 days after inoculation, showed advanced miliary tuberculosis of the lungs. Vaccinated heifer No.2 died of verminous bronchitis 5 months and 7 days after inoculation. No lesions resembling tuberculosis were found. Guinea pigs, inoculated with portions of, the apparently normal left bronchial lymph node, contracted tuberculosis. Vaccinated heifer No.1 remained in good condition and was butchered 12 months after inoculation. No lesions of tuberculosis could be found. Portions of the left bronchial lymph node were injected into 4 guinea pigs. These were killed but no indication of tuberculosis could be found in them. Group II. Vaccinated heifers 3 and 4 and control No. 14 were inoculated 3 months after vaccination. Control No. 14 died of miliary tuberculosis 44 days after inoculation. Vaccinated heifers 3 and 4 remained in good condition. They were slaughtered 11 months after the test inoculation and no tuberculous lesions could be found. Portions of their bronchial lymph nodes were inoculated into guinea pigs. These pigs were killed 45 days after inoculation. Those that had received tissue from heifer No.4 were tuberculous, but those inoculated from No.3 were free from tuberculosis. Calmette(12) in the latest edition of his book L'Infection Baeillaire ei lao tuberculose chez l.'Homme et chez les Animau.x attributes great importance to the observation that the guinea pigs inoculated from heifers 1 and 3 remained free from tuberculosis. In previous experiments he had observed that calves vaccinated by the intravenous route and later inoculated by the same route with virulent bacilli, remained free from caseous lesions, but their apparently normal lymph-node tissue would retain the bacilli virulent for guinea pigs for at least 18 months. The fact that guinea pigs did not contract tuberculosis when injected with tissues from heifers 1 and 3 was interpreted by Calmette to indicate that the subcutaneous injection of BCG is more efficacious than the intravenous method of vaccination. Group III. Vaccinated heifers Nos. 5 and 6 and control No. 15 were inoculated 6 months after vaccination. Control No. 15 became .very emaciated, was killed 60 days after inoculation and was found to 5 Only 19 heifers are accounted for in their descriptions.

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