Neurocysticercosis is a common disease in underdeveloped countries. Its diagnosis is based on clinical, imaging (tomography or magnetic resonance), epidemiological, and laboratory data. Several methods based on the detection of antibodies against cysticerci in cerebrospinal fluid or serum have been tested. Among them, an enzyme-linked immunosorbent assay (ELISA) based on the use of a crude parasite antigen has been used by the laboratory network of cysticercosis in Mexico, which has given support to clinicians for up to 7 years. A Taenia solium-specific glycoprotein-based electroimmunotransfer blot (EITB) assay was reported to be highly sensitive and specific for this purpose. In order to compare both techniques, we studied 100 neurocysticercosis patients and 70 neurological noncysticercosis controls and searched for specific antibodies in paired samples of serum and cerebrospinal fluid using both techniques. We found that the EITB assay is more sensitive than the ELISA, especially when serum is being tested. Both techniques are more sensitive in cases with multiple living cysts than in cases with single cysts or calcified lesions. No global differences among cases with parasites located in different parts of the central nervous system were found. In the patients with cysts within the parenchyma, the sensitivity of the EITB assay was higher with serum than with cerebrospinal fluid. The immunodominant bands were found to be the same as those previously reported, i.e., GP-39 to -42, GP-24, and GP-13. Based on these results, we suggest the use of the EITB assay in routine diagnosis of cysticercosis for clinical cases.Taeniasis and cysticercosis caused by Taenia solium, the pork tapeworm, are widespread infections in Latin America, Africa, and Asia (6). The disease in humans (neurocysticercosis [NC]) is caused by the metacestode, which develops within the central nervous system. It is often disabling and sometimes fatal. Diagnosis of cysticercosis is suggested by clinical, epidemiological, and serological findings (3), but magnetic resonance imaging or computed tomograpy (CT) scans are the most sensitive and specific diagnostic tools (14). To support them, several laboratory methods have been standardized, which are based on the detection of antibodies against cysticerci or parasite antigens in the cerebrospinal fluid (CSF) or the serum (2). Among them, the electroimmunotransfer blot (EITB) assay developed at Centers for Disease Control and Prevention (15) has the highest sensitivity in serum. This test develops up to seven glycoprotein bands which are specific for T. solium cysticercosis.In Mexico, NC was recognized as a problem of public health several years ago (7). The Central Laboratory of the National Network for Diagnosis of Cysticercosis (10) gives support to clinicians of second-and third-level hospitals of the country to diagnose this disease and to epidemiologists for field studies. The method that has been used up to now is an enzyme-linked immunosorbent assay (ELISA) which is based on the use of a crude...