The growth performance, body composition, fillet fatty acid content, serum hepatic enzymes and postprandial changes in serum lipid metabolism of hybrid sturgeon (70.8 ± 0.5 g) were investigated to determine the effects of total replacement of 80 g kg -1 fish oil (diet A) with linseed oil (diet B) and soybean oil (diet C), respectively. No significant differences in weight gain rate and specific growth ratio were observed among all fish groups (p > .05). Diet A fish group had the highest, but diet B fish group had the lowest feed intake (p < .05).
This paper studied the effects of increased carbon (C) and nitrogen (N) supplementation in pond production of Artemia franciscana cysts in southern Vietnam. Pig manure and tapioca were used to supplement the natural food of Artemia in the ponds at a fixed C/N ratio 10. Addition of pig manure and tapioca at a standard rate (0.210 kg ww and 0.319 kg dw pond(-1) day(-1), respectively) was used as control. Increasing amounts of supplementation (110, 115, and 120 % of the control) were applied in three treatments. During the 6-week culture period, extra C and N supplementation resulted in a better growth, faster maturation, and higher fecundity of Artemia when compared to the control. Total cyst production was the highest (9.96 kg ww ha(-1)) at the supplementation level 10 % higher than the control; the lowest cyst production was found in the control (2.84 kg ww ha(-1)). Increasing the supplementation of C and N at levels 110, 115, and 120 % of the control at C/N ratio 10 may have stimulated the formation of bioflocs in the hypersaline Artemia ponds and helped to improve conditions for increasing cyst yields
Biomass growth, ginsenoside and polysaccharide production in different ginseng tissue cultures, including callus culture, adventitious root culture and hairy root culture, were studied, and the active component contents were compared with that of native ginseng roots. The adventitious root culture was confirmed to be a very nice system, which grew fast and contained a rather high content of ginsenosides. Then, the culture conditions of adventitious root culture were optimized. The results showed that salt strength, various sucrose concentrations, ammonia/nitrate ratios and phosphate concentrations had significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in ginseng. The best culture conditions for ginsenoside production seemed to be 0.75 salt strength Murashige and Skoog medium, 4% sucrose, 9 mM ammonia to 36 mM nitrate, and 1.25 mM phosphate, while the optimization for polysaccharide accumulation seemed to be 0.75 salt strength, 6% sucrose, 9 mM ammonia to 36 mM nitrate and 3.75 mM phosphate source. Appropriate conditions allowed for a maximum ginsenoside yield of up to 132.90 mg/L and polysaccharide yield of 407.63 mg/L to be obtained after 4 weeks of culture.
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