Both static and dynamic strain effects of La0.7Ce0.3MnO3 (LCeMO) thin films that were grown on (001) oriented SrTiO3 (STO) and 0.7Pb(Mg1/3Nb2/3)O3-0.3PbTiO3 (PMN-PT) substrates were investigated. A charge-orbital ordering (COO) transition was found in the region of low temperature for strained (001)-LCeMO/STO films. The gradual vanishing of the COO transition with the relaxation of tensile epitaxial strain indicates the key role of tensile strain in the formation of COO. Furthermore, the results of dynamic strains on (001)-LCeMO/PMN-PT film show that even a small strain modification of ∼ −0.1% could evidently change the magnetotransport properties around TCOO, verifying the dominant role of the tensile strain.