A juvenile offshore bottlenose dolphin (Tursiops truncatus) was found stranded with neurological signs and unable to swim or float unassisted. It subsequently died, succumbing to a combination of severe pneumonia and encephalitis. Morbillivirus serum neutralisation test serology was positive (titre 1:16) for cetacean morbillivirus and negative for both phocine distemper virus and canine distemper virus. There was concurrent thymic and lymph node lymphoid depletion and necrosis, together with intranuclear and intracytoplasmic acidophilic viral inclusion bodies and multinucleate syncytia within multiple organs. Paramyxovirus capsids were identified in lung sections via electron microscopy and morbillivirus antigen was demonstrated within sections of lung, thymus and brain by immunohistochemistry. Reverse transcription-polymerase chain reaction for morbillivirus nucleoprotein (N) and phosphoprotein (P) genes were positive and phylogenetic gene product sequence analysis revealed 98% and 94% sequence identity to dolphin morbillivirus, respectively. To the authors' knowledge, this is the first report of a cetacean mortality due to morbillivirus infection occurring in the southern hemisphere. Morbillivirus infection should be included in the differential diagnosis of stranded live or dead cetaceans in Australian waters, particularly if animals display neurological signs.

Recent advancements in DNA sequencing methodologies and sequence data analysis have revolutionised research in many areas of biology and medicine, including veterinary infection biology. New technology is poised to bridge the gap between the research and diagnostic laboratory. This paper defines the potential diagnostic value and purposes of next-generation sequencing (NGS) applications in veterinary infection biology and explores their compatibility with the existing validation principles and methods of the World Organisation for Animal Health. Critical parameters for validation and quality control (quality metrics) are suggested, with reference to established validation and quality assurance guidelines for NGS-based methods of diagnosing human heritable diseases. Although most currently described NGS applications in veterinary infection biology are not primary diagnostic tests that directly result in control measures, this critical reflection on the advantages and remaining challenges of NGS technology should stimulate discussion on its diagnostic value and on the potential to validate NGS methods and monitor their diagnostic performance.

Deep galaxy surveys have revealed that the global star formation rate (SFR) density in the Universe peaks at 1≤ z ≤2 and sharply declines towards z = 0. But a clear picture of the underlying processes, in particular the evolution of cold atomic (∼100 K) and molecular gas phases, that drive such a strong evolution is yet to emerge. MALS is designed to use MeerKAT's L-and UHF-band receivers to carry out the most sensitive (N(H I)>10 19 cm −2 ) dust-unbiased search of intervening H I 21-cm and OH 18-cm absorption lines at 0 < z < 2. This will provide reliable measurements of the evolution of cold atomic and molecular gas cross-sections of galaxies, and unravel the processes driving the steep evolution in the SFR density. The large sample of H I and OH absorbers obtained from the survey will (i) lead to tightest constraints on the fundamental constants of physics, and (ii) be ideally suited to probe the evolution of magnetic fields in disks of galaxies via Zeeman Splitting or Rotation Measure synthesis. The survey will also provide an unbiased census of H I and OH absorbers, i.e. cold gas associated with powerful AGNs (>10 24 W Hz −1 ) at 0 < z < 2, and will simultaneously deliver a blind H I and OH emission line survey, and radio continuum survey. Here, we describe the MALS survey design, observing plan and the science issues to be addressed under various science themes.