This study evaluated the prevalence of nontuberculous mycobacterium (NTM) in relation to the total number of cases of mycobacterial infections detected in patients admitted at the University Hospital of Santa Maria from 2008 to 2010. From the positive samples for the genus Mycobacterium, 67% belonged to the Mycobacterium tuberculosis complex (MTBC) and 33% of them were classified as NTM. This investigation aims to contribute to the epidemiology of mycobacterioses, inasmuch as patients infected by NTM require distinctive treatment and monitoring in comparison with those infected by MTBC.
The history of muscle biopsy dates back to 1860, when Duchenne first performed a biopsy on a patient with symptoms of myopathy (1) . Since then, the basic and clinical science of muscle and muscle disease has gone through three stages of development: the classical period, the modern stage and the molecular era.
This study aims to compare the frequency of Staphylococcus aureus nasal carriage among students from a Portuguese higher health school. Antimicrobial susceptibility testing was also assayed in order to detect methicillin-resistant S. aureus (MRSA) strains among the isolates. Nasal swabs and fingerprints from 60 healthy nursing and pharmacy students were collected, followed by inoculation and incubation at 37 degrees C for 24 h. All suspected S. aureus isolates were identified by routine laboratory procedures. The susceptibility to antimicrobial agents (tetracycline, gentamicin, chloramphenicol, amoxicillin/clavulanic acid, trimethoprim/sulphamethoxazole, oxacillin and vancomycin) of confirmed isolates was determined by a disc-diffusion method. Results showed 41.7% S. aureus colonisation among participants, and that the difference between nursing and pharmacy students was statistically significant. Antibiotic susceptibility testing demonstrated that S. aureus isolates showed variable sensitivity to antibiotics but, most importantly, were resistant to oxacillin and vancomycin. Although the frequency and prevalence of colonisation found is within the range previously described in healthy populations, increased resistance to antimicrobials and higher prevalence of MRSA among the student community was found.
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