Sudeck's critical point at the rectosigmoid junction is described as the point of origin of the last sigmoid arterial branch, originating from the inferior mesenteric artery (IMA). There is controversy on the importance of Sudeck's point, and the frequency in which the anastomosis is found. Furthermore, the diameter of the anastomosis, if present, may also impact on the viability of the caudal stump. This study aimed to determine the frequency in which a macroscopic anastomosis occurs, between the superior rectal artery and the last sigmoidal branch, in a cadaver population; the diameter of this anastomosis and the distance from the origin of the IMA to Sudeck's point. Sixty‐four cadavers were included in the study, excluding those with previous surgery to the rectosigmoid junction. Sudeck's point was carefully identified and dissected to establish the presence of an anastomosis. Subsequent measurements were performed using a digital caliper (accuracy = 0.01 mm). A macroscopic anatomosis was absent in three cases (4.7%). The mean diameter of the anastomosis when present was 1.9 mm (SD: 0.5 mm), and the distance from the origin of the IMA to Sudeck's point was 55.5 mm (SD: 14.6 mm). Although an anastomosis is present in the majority of cases, the vessel is very small in diameter, and may not be sufficient to meet the demands of the caudal stump. The distance from the origin of the IMA to Sudeck's point is sufficient enough to allow for ligation of the IMA proximal to Sudeck's point. Clin. Anat. 20:424–427, 2007. © 2006 Wiley‐Liss, Inc.

BackgroundLannea schweinfurthii, Zanthoxylum capense, Scadoxus puniceus and Crinum bulbispermum are used traditionally to treat neurological disorders. The aim of this study was to evaluate the cytoprotective potential of the four plants, after induction of toxicity using rotenone, in SH-SY5Y neuroblastoma cells.MethodsCytotoxicity of the plant extracts and rotenone was assessed using the sulforhodamine B (SRB) assay. Fluorometry was used to measure intracellular redox state (reactive oxygen species (ROS) and intracellular glutathione content), mitochondrial membrane potential (MMP) and caspase-3 activity, as a marker of apoptotic cell death.ResultsOf the tested plants, the methanol extract of Z. capense was the least cytotoxic; LC50 121.3 ± 6.97 μg/ml, while S. puniceus methanol extract was the most cytotoxic; LC50 20.75 ± 1.47 μg/ml. Rotenone reduced intracellular ROS levels after 24 h exposure. Pre-treating cells with S. puniceus and C. bulbispermum extracts reversed the effects of rotenone on intracellular ROS levels. Rotenone exposure also decreased intracellular glutathione levels, which was counteracted by pre-treatment with any one of the extracts. MMP was reduced by rotenone, which was neutralized by pre-treatment with C. bulbispermum ethyl acetate extract. All extracts inhibited rotenone-induced activation of caspase-3.ConclusionThe studied plants demonstrated anti-apoptotic activity and restored intracellular glutathione content following rotenone treatment, suggesting that they may possess neuroprotective properties.

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Type 2 diabetes mellitus is on the increase worldwide. Current treatments possess undesirable side-effects and therefore investigations into alternative remedies, which may be cost-effective and devoid of such side-effects, are ongoing. Aqueous and methanol extracts of the bark of Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro (Anacardiaceae) and Ziziphus mucronata Willd. subsp. mucronata (Rhamnaceae) were subjected to testing in order to assess their in vitro anti-diabetic activity. Total polyphenolic content and antioxidant activity, as well as the effect on α-amylase, α-glucosidase and cytotoxicity, were measured. Furthermore, glucose uptake was evaluated in C2C12 myotubes, 3T3-L1 adipocytes and HepG2 hepatocarcinoma cells, and insulin secretion in RIN-m5F rat pancreatic β-cells. The extracts of both plants inhibited the activities of α-amylase and α-glucosidase in a concentration dependent manner, with results being comparable with the positive control, acarbose. All the extracts contained antioxidant activity, with the methanol extracts of S. birrea displaying the strongest free radical scavenging capacity (IC 50 = 2.16 µg/mL). Most of the extracts were not cytotoxic to the cell lines with IC 50 values > 100 µg/mL. Extracts of both plants significantly increased glucose uptake in C2C12, 3T3-L1 and HepG2 cells. However, insulin secretion from RIN-m5F cells was not altered. This study provides evidence that the plants possess in vitro anti-diabetic properties.

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