On golf courses planted to creeping bentgrass, invasion of annual bluegrass is a constant concern. To analyze if nitrogen fertilization manipulation could bias growth to creeping bentgrass, both grasses were fertilized either through foliar or soil application with either urea or ammonium sulfate and the impact on shoot and root growth measured. Ammonium sulfate resulted in greater overall growth for both species. Foliar application resulted in greater shoot growth for annual bluegrass and soil application resulted in greater root growth for creeping bentgrass. Leaf samples, as well as multiple leaf samples collected from golf courses, were examined microscopically for potential routes for foliar nutrient uptake: stomata and aqueous pores. No statistical difference was observed in the stomatal number between the two species but annual bluegrass possessed more aqueous pores. The enhanced ability of annual bluegrass to benefit from foliar fertilization may aid in its encroachment on highly managed golf greens.

Perennial ryegrass (Lolium perenne) is the most cultivated cool-season grass worldwide with crucial roles in carbon fixation, turfgrass applications, and fodder for livestock. Lolium perenne forms a mutualism with the strictly vertically transmitted fungal endophyte, Epichloë festucae var lolii. The fungus produces alkaloids that protect the grass from herbivory, as well as conferring protection from drought and nutrient stress. The rising concentration of atmospheric CO2, a proximate cause of climatic change, is known to have many direct and indirect effects on plant growth. There is keen interest in how the nature of this plant–fungal interaction will change with climate change. Lolium perenne is an obligately outcrossing species, meaning that the genetic profile of the host is constantly being reshuffled. Meanwhile, the fungus is asexual implying both a relatively constant genetic profile and the potential for incompatible grass–fungus pairings. In this study, we used a single cultivar, “Alto”, of L. perenne. Each plant was infected with one of four strains of the endophyte: AR1, AR37, NEA2, and Lp19 (the “common strain”). We outcrossed the Alto mothers with pollen from a number of individuals from different ryegrass cultivars to create more genetic diversity in the hosts. We collected seed such that we had replicate maternal half-sib families. Seed from each family was randomly allocated into the two levels of the CO2 treatment, 400 and 800 ppm. Elevated CO2 resulted in an c. 18% increase in plant biomass. AR37 produced higher fungal concentrations than other strains; NEA2 produced the lowest fungal concentrations. We did not find evidence of genetic incompatibility between the host plants and the fungal strains. We conducted untargeted metabolomics and quantitative proteomics to investigate the grass-fungus interactions between and within family and treatment groups. We identified a number of changes in both the proteome and metabalome. Taken together, our data set provides new understanding into the intricacy of the interaction between endophyte and host from multiple molecular levels and suggests opportunity to promote plant robustness and survivability in rising CO2 environmental conditions through application of bioprotective epichloid strains.

Water‐use restrictions during the summer of 2016 in Guelph, ON, Canada, prevented the irrigation of natural turf soccer fields and provided a unique opportunity to study the effects on soil volumetric water content and surface hardness on actively used youth soccer fields. Soil volumetric water content and surface hardness were tested on a weekly basis from July through September 2016. Areas of the turf that became brown were compared with the areas that remained green. Surface hardness within areas of brown turfgrass cover frequently exceeded 100g (level of concern for player safety) even though soil volumetric water content was similar between the areas of green and brown turfgrass cover. Therefore, irrigation of sports fields during times of drought is recommended to preserve the cushioning effect of green turfgrass cover.