Large grazers are essential for nature conservation. In order to prevent grazers from moving to unintended areas, it may be necessary to keep them inside enclosures. Physical fences present a number of problems, such as fragmenting the landscape. Virtual fencing, however, is a possible replacement for physical fencing, making it possible to enclose grazers without physical boundaries. Virtual fencing systems utilise collars with GPS technology to track animals and deliver auditory warnings and electric impulses to keep animals within predefined boundaries. This study examines how effective the virtual fencing system Nofence© is at enclosing calves in a holistically managed setting. Holistic management is a rotational grazing technique where an enclosure is grazed in small strips at a time. It is investigated whether the calves become habituated to the virtual fence and whether there is a correlation between the number of warnings received by every two calves in order to explore potential herd behaviour. Finally, this study examines which calves interact the most with the virtual fence by investigating the relationship between physical activity and number of interactions. Seventeen calves were fitted with a GPS collar from the company Nofence© and placed in a holistically managed enclosure. Data were gathered from 4 July to 30 September 2022. The study found that virtual fence was able to contain calves inside the designated enclosure, and over time the calves received notably fewer electrical impulses compared to auditory warnings. The results of Pearson’s correlation between auditory warnings received by two random calves were inconclusive, but the use of a sliding window analysis should be further explored. Lastly, the most physically active animals were the ones who received the most auditory warnings, but they did not receive more impulses. No significant correlation was found between the number of electric impulses received and the physical activity of the animals.