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The synthesis of the poly(N-isopropylacrylamide-co-2-hydroxypropylmethacrylate) hydrogel along with the analysis of the residual monomers content and influence of monomer and crosslinker molar ratios on the swelling behaviour was investigated. Synthesis of thermosensitive hydrogel based on N-isopropylacrylamide was carried out with the molar ratios of 5, 10, 15 and 20 mol. % of monomer 2- hydroxypropyl-methacrylate, in the presence of ethylene glycol dimethacrylate as a crosslinker (1, 1.5, 2 and 3 mol. %) and 2,2'-azobis(2-methylpropionitril) as an initiator in acetone. The quantities of residual monomers in the synthesized copolymers were determined by HPLC method, ranging from 0.19 to 0.49 % for N-isopropylacrylamide and from 0.13 to 0.63 % for 2-hydroxypropyl-methacrylate, counting the amount of xerogel. The hydrogels swelling ratio depending on time at 20 and 40°C was examined. It was found that hydrogel with 5 mol. % 2-hydroxypropyl-methacrylate and 1 mol. % ethylene glycol dimethacrylate had the highest degree of swelling (α = 29.59) at 20°C, and that hydrogel with 20 mol. % 2-hydroxypropyl-methacrylate and 3 mol. % ethylene glycol dimethacrylate had the lowest swelling degree (α = 2.17) at 40°C

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ABSTRACT:In this work the synthesis of a crosslinked macroporous copolymer was effected from methyl methacrylate and acrylamide. The synthesis process began with emulsion prepolymerization, followed by sol-gel copolymerization until a hard block was obtained. Determination of the properties of the obtained material was carried out by FTIR, mercury porosimetry, and SEM microscopy. The material was characterized by a porous structure with open pores. The macroporous copolymer obtained can be used for polymer-analog reactions and the transformation of amide and ester groups into acyl azide groups. It can be used as a hard inert support for the immobilization of enzymes, or other proteins, by condensation of acyl azide group on polymer with the free amino group from the base amino acid of enzyme/protein. For the immobilization of microorganisms it can be used by vacuum diffusion of microorganism suspension into the porous structure, without active group transformation reactions. With microorganisms in the polymer pores, microorganism colonies form within the copolymer by microbial fermentation.