Introduction: Nosocomial (also known as hospital-acquired) infections are understood as infections which develop during patient's stay in hospital, but are not the direct cause of hospitalisation. The aim of the study: The purpose of the paper was to determine the knowledge of the nursing personnel from Świętokrzyskie Voivodeship on nosocomial infections, prevention and methods of combating them. Other addressed issues included the analysis of the work yeas and education level as related to the state of nurses' knowledge on nosocomial infections, and what are the reasons for insufficient prevention of diseases. Material and methods: The study included 208 nurses from Świętokrzyskie Voivodeship. Out of 208 questionnaires, 180 were completed correctly, the remaining 28 were answered incorrectly or incompletely,

The morbidity and mortality caused by endometrial cancer (EC) is still rising worldwide. In recent years, a new system of tumor stratification has been proposed based on POLE-mutational status, TP53, and microsatellite stability status. The aim of the study was to analyze a vast panel on the genes potentially involved in the genesis of endometrial cancer in the Polish population. One hundred and three white female patients with confirmed endometrial cancer were enrolled on the study. We performed sequencing using the Hot Spot Illumina panel and microsatellite stability with immunohistochemistry. We confirmed a key role of the TP53 mutation in progress to high-grade EC and parallelly some role of FGFR2 mutation. Moreover, our data present a vast landscape of mutations in EC and their polymorphism. We reported the meaning of FGFR2 mutation and TP53 (high copy number) in high-grade ECs. Our observation in MSI contribution is comparable with other studies. Finally, we see a strong need for the implementation of the TCGA classification.

Streszczenie Wizyta u ginekologa nie przychodzi kobiecie łatwo i związana jest z obawami, które przeradzają się w strach, lęk i niepokój. Kobiety oczekują od swojego lekarza delikatności, dyskrecji oraz komunikatywności. Większość kobiet wybierając lekarza korzysta z opinii osób, które to doświadczenie mają już za sobą. Celem pracy było poznanie powodów, dla których kobiety zgłaszają się na wizyty do ginekologa oraz przedstawienie ich w aspekcie psychologicznym, diagnostycznym i leczniczym. Badania przeprowadzono w latach 2011-2012 na 403 studentkach z województwa świętokrzyskiego i mazowieckiego, w przedziale wiekowym 17-47 lat, by poznać powody wizyt kobiet w gabinecie ginekologicznym oraz ich oczekiwania. W tym celu przeprowadzono badanie ankietowe. Pytania wymagały od ankietowanych odpowiedzi tak lub nie, albo zaznaczenia kilku odpowiedzi. Na pytania odpowiadały kobiety zamieszkujące tereny wiejskie i miejskie. Warunkiem włączenia do badania była już odbyta wizyta u ginekologa. Wyniki badań wykazały, że: wizyta u ginekologa wiąże się ze stresem, który wywołany jest obawami, wstydem i strachem przed bólem lub wykryciem choroby. Coraz więcej kobiet i w coraz młodszym wieku zgłasza się o poradę do ginekologa. Większość kobiet, mimo obaw przed pierwszą wizytą, do kolejnych przystępuje z większą odwagą, a ich odczucia zamieniają się w pozytywne. Najbardziej oczekiwaną przez kobiety cechą jest komunikatywność lekarza oraz jego pozytywne nastawienie do pacjentki. Miejsce zamieszkania nie ma wpływu na świadomość kobiet zagrożenia chorobami przenoszonymi drogą płciową.

Background. At present, EC staging is based on the WHO conservative criteria, which only consider the percentage of gland formation. The molecular subgrouping of EC recently proposed by the Cancer Genome Atlas (TCGA) represents a milestone in precise molecular-based patient triage. The present study aimed to investigate the influence of FGFR-2 on the epithelial–mesenchymal transition (EMT) and whether it can lead to endometrial cancer dedifferentiation. Methods. One hundred and three White female patients with confirmed EC were enrolled in our research. For the analysis, we performed next-generation sequencing and immunohistochemical analyses of E-cadherin, β-catenin, and vimentin. Results. Tumor grade progression was closely correlated with LVI (p = 0.0338), expression of vimentin (p = 0.000), tumor budding (p = 0.000), and lack of E-cadherin (p = 0.0028). Similar observations were noted with regard to TNM/FIGO stage progression. In terms of FGFR-2 mutation, we found the following correlation p-values: LVI (p = 0.069), expression of vimentin (p = 0.000), tumor budding (p = 0.000), and lack of E-cadherin (p = 0.000), RFS (p = 0.032), ECSS (p = 0.047). Conclusions. FGFR-2 is the important factor influencing on EMT.

Patient: Male, newborn Final Diagnosis: ARCS1 Symptoms: Cholestasis and/or gallbladder dysfunction Medication: — Clinical Procedure: — Specialty: Pediatrics and Neonatology Objective: Rare disease Background: ARCS1 is an acronym for arthrogryposis, renal dysfunction, and cholestasis. It is a congenital malfunction with autosomal recessive inheritance, and, unfortunately, its prognosis is still poor. It is believed that VPS33B is altered in 75% of cases and that the VIPAR gene is altered in approximately 25% of them. Case Report: An affected child was born from the first pregnancy of 26-year-old mother and a 30-year-old father with no previous medical history and no genetic conditions. The first clinical symptoms were observed at the end of the child’s second week of life. The mother reported the child has decreasing body weight and loss of appetite. After admission to the ward, the child was apathetic and sleepy. Symptoms of conjunctivitis, pale and dry skin, and mild face and mild body dysmorphia were observed. Conclusions: Laboratory tests revealed proteinuria of up to 1.36 g/l and glycosuria of up to 28 mmol/l, as well as fluctuating metabolic acidosis. The bilirubin level reached 6.62 mg/dl, along with alkaline phosphatase at 470 U/l. Moreover, hypothyroidism with TSH at 16.71 uU/ml was observed. Because of the co-occurrence of cholestasis and renal dysfunction, molecular testing was done. The 17 th exon of VPS33B was sequenced by Sanger DNA sequencing method. To the best of our knowledge, this is the first report of homozygotic mutation c.1235_1236delinsG (p.Pro412ArgfsTer7) in the VPS33B gene. The risk of transfer of the mutation to future descendants was calculated as 25%. Due to the wide landscape of molecular alternation in the 17 th exon of the VPS33B gene, we propose using Sanger whole-exon sequencing as a first-choice diagnostic test.