No abstract

In this study, a disease case caused with 15% mortality on gilthead sea bream (Sparus aurata) which was cultured in the Antalya Bay in the Mediterranean coast of Turkey was investigated in July 2015. Lethargy, loss of equilibrium, petechae on the operculums and the dorsal part of the body, pillar of the gills were recorded in the diseased fish. Internally, ascites, splenomegaly and hemorrhages on the intestinal tissue and muscle were observed. Occurrence of parasite was not detected. According to the results of phenotypical test and 16S rDNA sequencing analysis, the isolated bacterial species was identified as Staphylococcus hominis. Histopathologically, hyperplasia of primary lamellae, lamellar telangiectasia, edema and hemorrhages on the gill arch, numerous melanomacrophage centers (MMCs) in the spleen tissue, vacuoler degeneration, necrose and hemorrhages in the liver, multifocal necrosis, and numerous MMC in the kidney were determined and hemorrhages in the tunica propria region of the intestine were observed. It was determined that he isolates showed different susceptibility against antimicrobial agents.

In the study, antimicrobial resistance and plasmid profile of Vibrio alginolyticus strains isolated from diseased European sea bass (Dicentrarchus labrax, L.) collected from a commercial farm located on the Agean Region coast of Turkey, were investigated. The agent was identified by classical biochemical tests and the API 20NE system. Bacterial strains were tested for resistance to different antimicrobials by disc agar diffusion method and minimal inhibitory concentration (MIC) at two different incubation temperatures (22 ± 2ºC and 28 ± 2ºC for 24 to 28 h), and screened for plasmid DNA by agarose gel electrophoresis. It was found that 15 strains were susceptible to kanamycin but they revealed resistance to ampicillin, bacitracin and streptomycin at 22ºC. The strains were resistant to bacitracin and streptomycin but 14 strains were found to be intermediately resistant. One strain was resistant to ampicillin at 28ºC; two strains showed sensitivity to kanamycin, whereas 12 strains were intermediately resistant, and one strain was resistant to the same antibiotic at 28ºC. The strains demonstrared susceptibility to low concentrations of chloramphenicol, kanamycin, and trimethoprim (MICs of ≤ 8 μg/mL) at 22ºC. The examined strains were found to be susceptible to chloramphenicol (MICs of ≤8 μg/mL) and trimethoprim (MICs of ≤8 μg/ mL) at 28ºC. The strains harboured 2-3 plasmids, with sizes ranging from 68 to 126 kb.

Antalya Körfezi'nde şimdiye kadar Lagocephalus spadiceus, Lagocephalus suezensis, Lagocephalus sceleratus, Torquigener flavimaculosus, Sphoeroides pachygaster, Tylerius spinosissimus olmak üzere 6 tür balon balığı tespit edilmiştir. Gazipaşa'da 09.01.2018 tarihinde yapılan dip paraketası avcılığı sırasında (160 m derinlikte) farklı bir tür balon balığı türü daha yakalanmış ve Mavi Balon Balığı ismiyle bilinen Lagocephalus lagocephalus (Linnaeus, 1758) olarak tayin edilmiştir.

The aim of this study is to inform isolations of Shewanella putrefaciens, Aeromonas sobria and Aeromonas veronii from the silver European eel fish (Anguilla anguilla) caught from the Gulf of Antalya, Turkey. Three silver eel fish samples as freshly dead (mean weight 75 g) were obtained by a local fisherman. For presumptive identification of the bacterial strains, the strains were identified using a variety of phenotypic characteristics. The further identification of the strains was performed with PCR. Clinically, the silver eel samples showed pale gills, losses of scale, necrotic lesions on dorsal, hemorrhagies on the jaws, lateral and ventral sides of the body and around the anus and hemoragic anal fin. At necropsy, the fish had enlarged and pale spleen and the gastrointestinal tract was empty. After 72 hours, 10 bacterial strains were isolated. 6 strains as Shewanella putrefaciens, 2 strains as Aeromonas sobria and 2 strains as Aeromonas veronii were identified according to the phenotypical characteristics and PCR study results. The eel fish stocks in natural conditions are decreasing and this problem may be multifactorial such as disease, migration and overhunting. As a result of this study, Aeromonas veronii was first isolated from Anguilla anguilla.