This study was undertaken to determine whether there was genetic diversity among isolates of Botrytis cinerea (teleomorph Botryotinia fuckeliana) collected from greenhouses and field locations in Alberta, Canada, and whether genetic diversity could be attributed to collection location and (or) host plant species. The study was conducted to provide basic information that is intended for use in developing effective greenhouse control strategies for B. cinerea. Ninety-one isolates of B. cinerea were collected from 20 host plant species in 31 greenhouse and field locations across the province. Relationships among subsets of the isolates were assessed, using random amplified polymorphic DNA (RAPD) analysis, and correlated with collection location and host plant. The first subset comprised 38 isolates collected from tomato (Lycopersicon esculentum) in eight greenhouses. The second subset was made up of 50 isolates from 10 host species collected in 10 greenhouses. The third subset consisted of 35 isolates from 17 host species from 23 greenhouses and 8 field locations. Random amplified polymorphic DNA analysis demonstrated distinct groups of B. cinerea within greenhouses in Alberta. Isolates in the three subsets clustered together based on the greenhouse of origin, but not on geographic region or host species. To our knowledge this is the first report of specialization in B. cinerea based on the greenhouse of origin.Résumé : La présente étude a été entreprise afin de déterminer s'il y avait de la diversité génétique parmi les isolats du Botrytis cinerea (téléomorphe Botryotinia fuckeliana) prélevés dans des serres ou des champs de l'Alberta, Canada, et si la diversité génétique était dépendante du site de prélèvement et de l'espèce de plante hôte. Le but de l'étude était de fournir une information de base pour utilisation dans le développement de stratégies efficaces de lutte en serre contre le B. cinerea. Quatre-vingt onze isolats de B. cinerea ont été prélevés sur 20 espèces de plantes hôtes dans 31 sites en serre ou au champ répartis dans la province. L'étude des liens entre les sous-ensembles d'isolats a été faite par analyse de l'ADN polymorphe amplifié au hasard et par comparaison entre les sites de prélèvement et les plantes hôtes. Le premier sous-ensemble comprenait 38 isolats prélevés de tomates (Lycopersicon esculentum) dans huit serres. Le second sous-ensemble était composé de 50 isolats de 10 espèces hôtes prélevés dans 10 serres. Le troisième sousensemble comprenait 35 isolats de 17 espèces hôtes provenant de 23 sites en serres et de 8 en champ. L'analyse de l'ADN polymorphe amplifié au hasard a révélé des groupes distincts de B. cinerea au sein des serres en Alberta. Les isolats des trois sous-ensembles se regroupaient en fonction de leur serre d'origine, mais non en fonction de la région géographique ou de l'espèce hôte. À notre connaissance, c'est la première fois que l'on signale la spécialisation du B. cinerea en fonction de la serre d'origine.
Her Majesty the Queen in Right of Alberta. All rights reserved.No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical photocopying, recording, or otherwise without written permission from the Information Packaging Centre, Alberta Agriculture, Food and Rural Development.
No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical photocopying, recording or otherwise without written permission from the Information Packaging Centre, Alberta Agriculture, Food and Rural Development.
This chapter focuses on Botryotinia fuckeliana [Sclerotinia fuckeliana], the causal agent of grey mould or Botrytis blight in greenhouse crops, including vegetables, bedding plants, potted plants and perennials in Canada. It provides an overview of the fungi (Trichoderma spp.) and bacteria (Streptomyces spp.) that have been used for its management. Future research prospects are recommended.
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