Enzyme additives are believed to improve septic tank performance by increasing the hydrolysis and digestion rates and maintaining a healthy microbial population. Previous studies reported mixed results on the effectiveness of enzymes on mesophilic and thermophilic digestion, and it is not clear whether enzymes would be effective under septic tank conditions where there is no heating or mixing, quantities of enzymes added are small, and they can be washed out quickly. In this study, batch reactors and continuous-flow reactors designed and operated as septic tanks were used to evaluate whether enzymatic treatment would increase the hydrolysis and digestion rates in primary sludge. Total solids, volatile solids, total suspended solids, total and soluble chemical oxygen demand, concentrations of protein, carbohydrate, ammonia and volatile acids in sludge and effluent samples were measured to determine the differences in digestion rates in the presence and absence of enzymes. Overall, no significant improvement was observed in enzyme-treated reactors compared with the control reactors.

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Freeze-thaw conditioning of RBC (Rotating Biological Contactor) sludge was tested using a pilot-scale freezing bed placed in a mobile freezer operated at -10°C. Sludge samples from a remote mining exploration facility were flown in every 2 weeks, and added to the freezing bed in 8 layers of 10 cm thick. Approximately 4 months after the first layer of sludge was added, the pilot unit was removed from the freezer and thawed at ambient temperatures. After one day of thawing, the solids concentration increased from 2.6% to 16.2%. The final cake solids concentration was 21%. Melt water had increasing turbidity, COD, TSS, VSS, nitrogen and phosphorus concentrations during the thawing period. Freeze-thaw conditioning also decreased the initial densities of fecal coliforms and Salmonella in sludge. The results of this study showed that freeze-thaw technology successfully dewatered RBC sludge without the need for mechanical equipment, and is a sustainable option for sludge dewatering in cold and remote regions.

Micro-aeration, which refers to the addition of very small amounts of air, is a simple technology that can potentially be incorporated in septic tanks to improve the digestion performance. The purpose of this study was to investigate and compare the effects of micro-aeration on anaerobic digestion of primary sludge under septic tank conditions. 1.6 L batch reactor experiments were carried out in duplicate using raw primary sludge, with 4.1 % total solids, and diluted primary sludge, with 2.1 % total solids. Reactors were operated for 5 weeks at room temperature to simulate septic tank conditions. Micro-aeration rate of 0.00156 vvm effectively solubilised chemical oxygen demand (COD) and improved the subsequent degradation of COD. Micro-aeration also increased the generation of ammonia and soluble proteins, but did not improve the reduction in total and volatile solids, or the reduction in carbohydrates. Experiments using diluted sludge samples showed similar trends as the experiments with raw sludge, which suggest that initial solids concentration did not have a significant effect on the degradation of primary sludge under septic tank conditions.

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