Bioaerosol concentrations inside one naturally ventilated and one mechanically ventilated swine finishing barn were assessed by sampling air using membrane filtration and impaction (six-stage Andersen sampler), and assayed by culture method. The barns, located on the same commercial farm in northeast Kansas, did not show any significant difference (p > 0.05) in concentrations of total and respirable airborne microorganisms. The overall mean total concentrations inside the two barns were 6.6 x 10(4) colony forming units (CFU)/m3 (SD = 3.8 x 10(4) CFU/m3 as measured by filtration and 8.6 x 10(4) CFU/m3 (SD = 5.1 x 10(4) CFU/m3) by impaction. The overall mean respirable concentrations were 9.0 x 10(3) CFU/m3 (SD = 4.1 x 10(3) CFU/m3) measured by filtration and 2.8 x 10(4) CFU/m3 (SD = 2.2 x 10(4) CFU/m3) by impaction. Total and respirable CFU concentrations measured by impaction were significantly (p < 0.05) higher than that by filtration. The persistent strains of microorganisms were various species of the following genera: Staphylococcus, Pseudomonas, Bacillus, Listeria, Enterococcus, Nocardia, Lactobacillus, and Penicillium. It appears that filtration sampling can be used for a qualitative survey of bioaerosols in swine barns while the Andersen sampler is suitable for both quantitative and qualitative assessments.

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When Escherichia coli 15T-cells growing exponentially at 70to 80-min doubling times are subjected to a nutritional shift-up via glucose addition, cell division continues at the preshift rate for about 70 min (rate maintenance). The same cells growing at doubling times of 120 min or longer, however, begin to divide at a new faster rate immediately upon glucose addition. In both the rate maintenance and immediate division situations, cell mass, as measured by optical density (OD), begins to increase immediately upon shift-up. Consequently, the OD/cell pattern differs in the two growth-rate transitions. During rate maintenance, the OD/cell ratio increases dramatically for 60 to 70 min, and then slows appreciably and approaches the OD/cell characteristic of the new medium. During immediate division situations, the OD/cell increases only