When immersed in a non-uniform electrolyte solution, a rigid charged sphere migrates toward higher or lower concentration of the electrolyte depending on the relative ionic mobilities and the charge borne by the sphere. This motion has a twofold origin: first, a macroscopic electrolyte gradient produces an electric field which acts on the charged sphere (electrophoresis); secondly, the electrolyte gradient polarizes the cloud of counterions surrounding the charged sphere by making the cloud thinner on the high-concentration side (chemiphoresis). In this paper, we compute the terminal velocity of a non-conductive sphere through a slightly non-uniform solution of a symmetrically charged binary electrolyte. The analysis proceeds through an expansion in the small parameter λ (defined as the ratio of the counterion-cloud thickness to the particle radius). Results to O(λ) are presented. The only property of the sphere's surface that affects the velocity is its zeta potential ζ when the electrolyte gradient vanishes; no information concerning the dependence of ζ upon ionic strength is needed. While the chemiphoretic effect always directs the particle toward higher electrolyte concentration, the electrophoretic contribution can move the particle in either direction depending on the sign of βζ, where β is a normalized difference in mobilities between cation and anion of the elecytrolyte; thus particle movement could be directed toward either higher or lower electrolyte concentration depending on the physical properties of the system. With slight algebraic rearrangement, our results are also applicable to conventional electrophoresis (particle motion in an applied electric field) and show excellent agreement with the numerical calculations of O'Brien & White (1978).

be related to any physical quantity characterizing the interaction between adsorbates and adsorbents. We inferred

The skin forms a critical structural boundary and a perceptual interface for the organism, yet the definition "healthy skin" is surprisingly difficult to describe. The present study's goal was to generate a technical definition of healthy infant skin by quantifying specific biophysical parameters before and after bathing in infants and correlating such parameters to a perceptual maternal evaluation. Fifty-two healthy infants, 3-6 months old, were evaluated before and after freshwater bathing. Diapered skin had a higher transepidermal water loss (TEWL), surface hydration, moisture accumulation rate (MAT), and friction than nondiapered skin before the bath (p < 0.01). Bathing dramatically altered the biophysical properties at both skin sites, with decreased MAT and lower friction, indicating a drier skin surface (p < 0.01). Visual redness and dryness decreased after bathing (p < 0.01). Blinded grading of optical images showed a significant preference for the skin after bathing (p < 0.01). This study provides the first quantitative technical definition of healthy infant skin with positive correlation to perceptual assessment by independent observers (mothers). The findings support the hypothesis that water binding properties of the stratum corneum are altered by occlusion (diapering) and that bathing introduces acute changes in stratum corneum water interactions, leading to a drier skin surface and a preferred skin appearance.

Background/aims: Prolonged skin occlusion increases stratum corneum water content and often increases skin permeability and irritant dermatitis. As skin wetness from wearing diapers is considered an important factor favouring the onset of diaper dermatitis, optimal diapering might decrease skin hyperhydration and dermatitis. Our aim is to define the quantitative relationship between nicotinate ester (a model penetrant) skin permeability and hydration, as measured by water evaporation rate (WER), decay curves (at individual time points) and WER‐area under the curve (WER‐AUC); and also to determine the level of skin hydration and skin permeability to nicotinates following a diapering simulation. Methods/results: Nine healthy Caucasian adult women were enrolled after a prescreening procedure (time to peak redness response to nicotinate); each received three wet occlusive patches for different exposure times (10 min, 30 min, and 3 h) and two wet model diapers (3 and 8 h). Prior to patching or diapering of forearms, basal values of WER, skin blood flow volume (BFV), capacitance (Cap) and redness (a*) were measured on premarked sites (a, b, c and d). Immediately, following occlusive patch or diaper removal, 20 µL of each nicotinate (methyl and hexyl nicotinate) was applied to its respective site (a or b). The WER and Cap readings were recorded at designated sites (c and d) with the following intervals after nicotinate applications: 0, 5, 10, 15 and 20 min. The a* and BFV measurements were made on each nicotinate challenged site (a and b) with the following intervals after nicotinate applications: 5, 10, 15, 20, 30, 40, and 60 min. Results: WER‐AUC and thus, skin hyperhydration, increased with occlusive patch and diaper exposure time, but there was no statistical difference between 3 and 8 h diaper sites. All patched sites had significantly (P < 0.05) increased hydration in comparison to control sites (undiapered or unpatched skin). Cap increased with occlusion time with patches, but not with diapers. The degree and time‐course of redness from nicotinates did not vary with extent of skin hydration, but was significantly increased compared to non‐hydrated skin. BFV‐AUC did not show a significant increase between diapers at 3 and 8 h sites; the BFV‐AUC values varied on the patched sites, but some were significantly (P < 0.05) higher than control site. Conclusion: Wet patches and diapers increased skin hyperhydration proportional to exposure time. Permeation of nicotinates was increased for hydrated skin vs. control, even after only 10 min of patch exposure. For these model permeants, we found no evidence of increased permeation rates with increased hyperhydration, once a relatively low threshold of hyperhydration was achieved (e.g. that reached after a 10 min wet patch). The data showed no meaningful differences in permeation following either diapering simulation and also suggested that the WER‐AUC method was superior to capacitance for measuring the absolute extent of hyperhydration. We believe this is a suitable model f...

We have developed a method for quantifying the absorption of model fluorescent latex particles from the mouse small intestine into Peyer's patches, mesenteric lymph nodes, and spleen. The procedure combines a simple and exhaustive particle recovery technique with a highly sensitive particle counting technique. Mice were orally gavaged with fluorescent polystyrene latex suspensions, and at various time points Peyer's patches, normal absorptive small intestinal tissue, mesenteric lymph nodes, and spleen were collected. The tissue samples were solubilized using an aqueous potassium hydroxide and surfactant solution and particles were counted using a flow cytometer. Using this method we were able to detect and quantify small numbers of particles, measure the course of uptake and clearance, and determine the tissue distribution of absorbed particles. Data generated using this technique indicate that particle absorption depends on the dose level, particle size, and fed state of the animals.