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This study investigated the levels of homophobia before and after a single lecture on the topic in one undergraduate and two graduate social work classes. Using the Index of Attitudes Towards Homosexuals (IAH), pretest levels of homophobia were found to exceed the cutting point of 50 for 75% of the students in the undergraduate class. Although pretest-posttest mean scores were not found to be statistically significant, the percentage of students exceeding the IAH cutting score decreased in all three courses following a single lecture. The effect of two additional teaching units in the undergraduate course is discussed as well as recommendations for future research.